苍附导痰汤对肥胖型PCOS大鼠内分泌激素及miRNA-16和PDCD-4表达的影响

Study on the Effect of Cangfu Daotan Tang(苍附导痰汤)on Endocrine Hormones and the Expression of miRNA-16 and PDCD-4 in Obese PCOS Rats

目的:探讨苍附导痰汤对肥胖型多囊卵巢综合征(PCOS)大鼠内分泌激素及微小RNA(miRNA)-16和程序性细胞死亡因子4(PDCD-4)表达的影响。方法:将50只SPF级雌性SD大鼠随机分为5组,每组10只。阳性对照组大鼠灌胃格华止(0.43 g/kg),低剂量组大鼠灌胃低剂量苍附导痰汤(1.42 g/kg),高剂量组大鼠灌胃高剂量苍附导痰汤(5.68 g/kg),模型组和正常组大鼠灌胃等量生理盐水,各组均持续给药14 d。比较各组大鼠体质量,内分泌激素水平,miRNA-16和PDCD-4 mRNA表达,以及PDCD-4蛋白表达。结果:模型组、阳性对照组、低剂量组和高剂量组大鼠体质量均高于正常组(P<0.05);阳性对照组、低剂量组和高剂量组大鼠体质量均低于模型组(P<0.05),且呈剂量依赖性。模型组、阳性对照组、低剂量组和高剂量组大鼠血清E_(2)均水平低于正常组,而血清T、FSH和LH水平均高于正常组(P<0.05);阳性对照组、低剂量组和高剂量组大鼠血清E_(2)水平均高于模型组,而血清T、FSH和LH水平均低于模型组(P<0.05),且呈剂量依赖性。模型组、阳性对照组、低剂量组和高剂量组大鼠卵巢miRNA-16表达均低于正常组,而PDCD-4 mRNA表达高于正常组(P<0.05);阳性对照组、低剂量组和高剂量组大鼠卵巢miRNA-16表达均高于模型组,而PDCD-4 mRNA表达低于模型组(P<0.05),且呈剂量依赖性。模型组、阳性对照组、低剂量组和高剂量组大鼠卵巢PDCD-4蛋白相对表达量均高于正常组(P<0.05);阳性对照组、低剂量组和高剂量组大鼠卵巢PDCD-4蛋白相对表达量均低于模型组(P<0.05),且呈剂量依赖性。结论:苍附导痰汤可调节肥胖型PCOS大鼠内分泌激素水平,其机制可能与上调miRNA-16表达及下调PDCD-4表达有关。

Objective:To investigate the effect of Cangfu Daotan Tang on the expression of endocrine hormones,microRNA(miRNA)-16 and programmed cell death factor 4(PDCD-4)in obese polycystic ovary syndrome(PCOS)rats.Method:Totally 50 SPF level female SD rats were randomly divided into 5 groups,with 10 rats in each group.The rats were gavaged with Gehuazhi(0.43 g/kg)in positive control group,and low-dose Cangfu Daotan Tang(1.42 g/kg)and high-dose Cangfu Daotan Tang(5.68 g/kg)respectively in low-dose group and high-dose group.And rats were gavaged with an equal amount of physiological saline in model group and normal group.Each group was continuously administered for 14 days.The body weight,endocrine hormone levels,miRNA-16 and PDCD-4 mRNA expression,as well as PDCD-4 protein expression were compared.Results:The model group,positive control group,low-dose group,and high-dose group showed higher body weight than normal group(P<0.05);The positive control group,low-dose group,and high-dose group showed lower body weight than model group(P<0.05),and it was dose-dependent.The model group,positive control group,low-dose group,and high-dose group showed lower E_(2) levels than normal group(P<0.05),while higher levels of T,FSH,and LH than normal group(P<0.05);The positive control group,low-dose group,and high-dose group showed higher E_(2)levels than model group(P<0.05),while lower levels of T,FSH,and LH than model group(P<0.05),and there was a dose-dependent relationship.The model group,positive control group,low-dose group,and high-dose group showed lower expression of miRNA-16 mRNA than normal group,while higher expression of PDCD-4 mRNA than normal group(P<0.05);The positive control group,low-dose group,and high-dose group showed higher expression of miRNA-16 mRNA than model group(P<0.05),while lower expression of PDCD-4 mRNA than model group(P<0.05),and it was dose-dependent.The model group,positive control group,low-dose group,and high-dose group showed higher relative expression levels of PDCD-4 protein than normal group(P<0.05);The positive control group,low-dose group,and high-dose group showed lower relative expression levels of PDCD-4 protein than model group(P<0.05),and it was dose-dependent.Conclusion:Cangfu Daotan Tang can regulate the levels of endocrine hormones in obese PCOS rats,and its mechanism may be related to the up-regulation of miR-16 expression and down-regulation of PDCD-4 expression.