临床分析标本溶血对生化检验中AST、GPT、LDH、K^(+)的影响

Clinical Analysis of the Effects of Hemolysis on AST,GPT,LDH,K^(+)in Biochemical Tests

目的 临床分析标本溶血对生化检验中天门冬氨酸氨基转移酶(AST)、谷丙转氨酶(GPT)、乳酸脱氢酶(LDH)、钾离子(K^(+))的影响情况。方法 选取本院门诊部收治的健康体检者共计80例,时间跨度为2020年1月—2023年2月,将受检者血液标本分成2份,分别进行常规生化检测与引发溶血后的生化检测,对两种不同血清标本天门冬氨酸氨基、谷丙转氨酶、乳酸脱氢酶、钾离子、总胆固醇、碱性磷酸酶、甘油三酯、尿素氮、肌酐、血糖、血尿酸、总胆红素生化指标检测值进行对比观察,随后把标本血清溶血后再测定上述生化指标,分析溶血前后检验结果。结果 在溶血标本中天门冬氨酸氨基转移酶检测值、谷丙转氨酶检测值、乳酸脱氢酶检测值、钾离子检测值、总胆固醇检测值,相较于非溶血标本明显偏高,且碱性磷酸酶、总胆红素与非溶血标本相比明显偏低,差异有统计学意义(P<0.05)。结论 标本溶血容易造成生化检验的天门冬氨酸氨基转移酶、谷丙转氨酶、乳酸脱氢酶、钾离子、总胆固醇以及碱性磷酸酶检测结果出现异常,运用血清血红蛋白浓度校对,有较高的运用价值,可为临床医师提供重要的参考依据。

Objective To analyze the effects of hemolysis on aspartate aminotransferase(AST),alanine aminotransferase(GPT),lactate dehydrogenase(LDH)and potassium ion(K+)in biochemical tests.Methods A total of 80 healthy physical examination patients admitted to the outpatient department of our hospital were selected.The blood samples of the subjects were divided into two parts from January 2020 to February 2023,and routine biochemical detection and biochemical detection after hemolysis were performed,respectively.Total cholesterol,alkaline phosphatase,triglyceride,urea nitrogen,creatinine,blood glucose,blood uric acid,serum aspartate amino acid,alanine aminotransferase,lactate dehydrogenase,potassium ion,total cholesterol,alkaline phosphatase,triglyceride,urea nitrogen,creatinine of two different serum samples were analyzed.The biochemical indexes of total bilirubin were compared and observed,and then the above biochemical indexes were determined after the serum was hemolyzed,and the test results before and after hemolysis were analyzed.Results The values of aspartate aminotransferase,alanine aminotransferase,lactate dehydrogenase,potassium ion and total cholesterol in hemolytic specimens were significantly higher than those in non-hemolytic specimens,and alkaline phosphatase and total bilirubin were significantly lower than those in non-hemolytic specimens.The calculation results were statistically(P<0.05).Conclusion The results of aspartate aminotransferase,alanine aminotransferase,lactate dehydrogenase,potassium ion,total cholesterol and alkaline phosphatase in the biochemical test of hemolysis specimens are prone to be abnormal.The calibration by serum hemoglobin concentration has high application value and can provide important reference for clinicians.