评估胚胎发育潜能研究的新进展

Progress in the study of evaluating embryonic development potential

胚胎发育潜能关系到辅助生殖结局中单胚胎移植的选择和最终的临床妊娠结局。形态学评估法因其简单、便捷、廉价、较稳定和时效性高的优势,成为辅助生殖实验室评估胚胎发育潜能最广泛运用的方法;时差成像(TL)技术的诞生和兴起为辅助生殖实验室提供了实时记录胚胎完整发育动态过程的手段;代谢组学可通过检测葡萄糖、氨基酸、烟酰胺腺嘌呤二核苷酸(磷酸)、黄素腺嘌呤二核苷酸等代谢产物对胚胎发育潜能进行生理学上精确地评估;植入前遗传学检测(PGT)可对胚胎进行严重遗传疾病或染色体异常状况(非整倍性)的检测。本文对以上4种评估胚胎发育潜能方法的新进展进行综述。

Embryonic development potential will be related to the selection of single embryo transfer in assisted reproduction outcome and the final clinical pregnancy result.Because of its advantages of simplicity,convenience,low-priced,high stability and great efficiency,the morphological evaluation has become the most widely used method to evaluate embryonic development potential in assisted reproductive laboratory.The birth and rise of time-lapse(TL)technology provides a means for assisted reproductive laboratory to record the dynamic complete process of embryonic development in real time.Metabolomics study provides an accurate physiologically assessment of embryonic development potential by detecting metabolites such as glucose,amino acids,NAD(P)H and FAD+.The application of preimplantation genetic testing(PGT)tests embryos for severe genetic diseases or chromosomal abnormalities(aneuploidy).This article reviews the progress of the above four methods for evaluating embryonic development potential.