摘要
目的探究神经胶质瘤组织中微小RNA-203a-3p(miR-203a-3p)、配对同源异型盒蛋白2B(PHOX2B)表达水平与临床特征及预后的关系。方法选取2017年6月至2020年4月我院收治的96例神经胶质瘤患者,术中采集切除的胶质瘤组织及相应癌旁组织,采用qRT-PCR和免疫组化法检测胶质瘤组织和癌旁组miR-203a-3p、PHOX2B mRNA表达;采用Pearson法分析miR-203a-3p、PHOX2B mRNA的相关性;采用Kaplan-Meier生存曲线分析miR-203a-3p、PHOX2B mRNA与神经胶质瘤患者预后的关系;采用COX回归分析影响神经胶质瘤患者预后的危险因素。结果胶质瘤组织miR-203a-3p水平和阳性表达率显著低于癌旁组织(P<0.05),PHOX2B mRNA水平和阳性表达率显著高于癌旁组织(P<0.05)。Pearson相关性分析,神经胶质瘤患者miR-203a-3p、PHOX2B mRNA水平呈负相关(P<0.05)。miR-203a-3p、PHOX2B与KPS评分、肿瘤组织坏死和WHO分级有关(P<0.05)。Kaplan-Meier曲线分析得出,miR-203a-3p高表达组生存率显著高于低表达组(P<0.05),PHOX2B mRNA高表达组生存率显著低于低表达组(P<0.05)。COX回归分析表明,miR-203a-3p、PHOX2B mRNA、肿瘤组织坏死和WHO分级是影响神经胶质瘤患者预后的危险因素(P<0.05)。结论在神经胶质瘤中miR-203a-3p表达水平显著降低,PHOX2B mRNA表达水平显著升高,二者与临床特征及预后密切相关。
Objective To investigate the correlations microRNA-203a-3p(miR-203a-3p)and paired allographic box protein 2B(PHOX2B)expression levels in glioma tissues with clinical features and prognosis.Methods Ninety-six glioma patients admitted to our hospital from June 2017 to April 2020 were included in the study.The resected glioma tissues and corresponding paracancerous tissues from the patients were collected intraop⁃eratively.qRT-PCR and immunohistochemistry were conducted to detect the expression of miR-203a-3p and PHOX2B mRNA in glioma tissues and paracancerous groups.Pearson's method was used to analyze the correlation between miR-203a-3p and PHOX2B mRNA.Kaplan-Meier survival curves were used to analyze the correlations of miR-203a-3p and PHOX2B mRNA with the prognosis of glioma patients.COX regression was used to analyze the risk factors affecting the prognosis of glioma patients.Results The miR-203a-3p level and positive expression rate of glioma tissues were significantly lower than that of paracancerous tissues(P<0.05),and the PHOX2B mRNA level and positive expression rate were significantly higher than that of paracancerous tissues(P<0.05).By Pearson correlation analysis,the miR-203a-3p and PHOX2B mRNA levels of glioma patients were negatively correlated(P<0.05).miR-203a-3p and PHOX2B were associated with KPS score,tumor tissue necrosis,and WHO grading(P<0.05).Kaplan-Meier curve analysis yielded the results that the survival rate of the miR-203a-3p highly expressed group was significantly higher than that of the lowly expressed group(P<0.05),and the survival rate of the PHOX2B mRNA highly expressed group was significantly lower than that of the lowly expressed group(P<0.05).COX regression analysis showed that miR-203a-3p,PHOX2B mRNA,tumor tissue necrosis,and WHO grading were the risk factors affecting the prognosis of glioma patients(P<0.05).Conclusion The expression level of miR-203a-3p is obviously reduced and the expression level of PHOX2B mRNA is obviously increased in glioma,both showing close correlation with clinical characteristics and prognosis.
作者
张淇
胡嘉
李辉
王锦
李忠德
胡志源
ZHANG Qi;HU Jia;LI Hui;WANG Jin;LI Zhongde;HU Zhiyuan(Second Department of Neurosurgery,Zhangye People's Hospital,Hexi University,Zhangye 734000,China;不详)
出处
《实用医学杂志》
CAS
北大核心
2024年第10期1382-1388,共7页
The Journal of Practical Medicine
基金
甘肃省自然科学基金项目(编号:22JR5RG571)。