基于化学发光磁酶免疫技术建立粮食中铅的自动化检测方法

Establishment of an Auto-detection for Lead in Grains Based on Chemiluminescence Magnetic Enzyme Immunoassay Technology

利用抗原抗体高特异性,建立一种自动化的化学发光磁酶免疫方法,快速定量检测粮食中铅元素的含量。通过优化前处理条件和化学发光磁酶免疫分析方法的反应条件,实现了大米、小麦、玉米等粮食样品中铅的自动测定。该方法的检测范围为0.05~0.71 mg/kg,大米和玉米中检出限为0.02 mg/kg,小麦中检出限为0.01 mg/kg,回收率范围89.2%~113.6%,相对标准偏差均小于10%。基于化学发光免疫平台,本方法可在30分钟内完成8个样品的同时自动化检测,有效减少人为误差,提高检测准确性和检测效率,在粮食中铅的快速定量检测方面具有良好的应用前景。

An automatic chemiluminescence magnetic enzyme immunoassay based on the high specificity of antigen and antibody was developed for rapid and quantitative determination of lead in grain.By optimizing the pre-treatment conditions and the reaction conditions of chemiluminescence magnetic enzyme immunoassay,the automatic determination of lead in rice,wheat and corn samples was realized.The detection range was 0.05~0.71 mg/kg,and the limit of detection was 0.02 mg/kg in rice and corn and 0.01 mg/kg in wheat.The recovery range was 89.2%~113.6%,and the relative standard deviations were all less than 10%.Based on the chemiluminescence immune platform,the developed method can realize eight samples simultaneous auto-detection within 30 min.Meanwhile,the automatic detection method can avoid human errors,and greatly improve the accuracy and efficiency,showing a good application prospect in the rapid quantitative detection of lead in grain.