基于生物信息学、网络拓扑策略探究针刺治疗帕金森病的机制

Study of acupuncture’s mechanism in treating Parkinson’s disease based on bioinformatics and network topology strategies

目的运用生物信息学和网络拓扑策略,探究针刺治疗帕金森病(Parkinson’s disease,PD)的机制。方法系统检索中国知网、Pubmed等6个数据库中近10年针刺治疗PD的文献,筛选出代表性有效成分。通过STITCH和SwissTargetPrediction数据库获得其相关靶点。将有效成分靶点和疾病靶点取交集,基于STRING数据库,在Cytoscape软件制作蛋白相互作用图。再利用Metascape平台对核心靶点进行GO富集分析和KEGG富集分析。结果共筛选出针刺后人体产生变化的有效成分4个,针刺治疗PD核心靶点42个,GO富集分析涉及条目43条,KEGG分析涉及通路7条。结论针灸可能通过作用于TH、COMT、DRD2、MAOB等核心靶点,通过调节神经递质水平、突触功能、氧化应激系统等,从而达到治疗PD的目的。

Objective To discuss the mechanism of acupuncture in treating Parkinson’s disease(PD)using bioinformatics and network topology strategies.Method Six databases,including Chinese National Knowledge Infrastructure(CNKI)and Pubmed,were systematically searched for PD-related articles published during the past 10 years to collect typical active ingredients.STITCH and Swiss Target Prediction databases were used to obtain relevant targets.The STRING database and Cytoscape software were used to develop the protein-protein interaction(PPI)network based on the common ground between the effective ingredient targets and disease targets.GO and KEGG enrichment analyses of the core targets were conducted on the Metascape platform.Result Four active ingredients that altered after acupuncture in the human body and 42 core targets in the acupuncture treatment of PD were collected.The GO enrichment analysis involved 43 items,and the KEGG analysis involved 7 pathways.Conclusion Acupuncture may regulate the neurotransmitter level,synaptic function,and oxidative stress system via core targets like TH,COMT,DRD2,and MAOB to treat PD.