基于网络药理学探讨白芷调控自噬治疗色素沉着性疾病的物质基础

Material Basis of Angelica Dahurica's Regulation of Autophagy to Treat Melanin Deposition Diseases Through Network Pharmacology

目的利用生物信息学分析研究白芷有效成分靶基因及机制。方法通过中药系统药理学数据库与分析平台(TCMSP),分析白芷有效活性成分的靶基因;从综合数据库GeneCards中获取与黑色素相关的疾病靶基因,从芯片GSE114445中筛选色素沉着性疾病相关基因,取交集后获得白芷有效活性成分靶基因中与色素沉着性疾病相关的基因;从自噬数据库筛选自噬相关基因,与白芷有效活性成分靶基因中和色素沉着性疾病相关基因取交集,获得白芷有效活性成分靶基因中与色素沉着性疾病和自噬相关的基因;通过Metascape在线分析白芷有效活性成分靶基因中与色素沉着性疾病和自噬相关的基因富集通路。结果获得5342个白芷药靶基因,其中有152个和色素沉着性疾病相关基因,9个与色素沉着性疾病和自噬相关的基因;白芷有效活性成分靶基因中与色素沉着性疾病和自噬相关的基因主要富集在磷脂酰肌醇3激酶-蛋白激酶B(PI3K-Akt)、丝裂原激活的蛋白激酶(MAPK)、鼠类肉瘤病毒癌基因同源物(RAS)通路。结论筛选出的9个白芷药靶基因中与色素沉着性疾病和自噬相关的基因主要在PI3K-Akt、MAPK、RAS通路富集。本研究结果初步揭示了白芷药效的物质基础,为阐明白芷促进黑色素降解作用机制提供了参考。

Objective In order to study the target genes and mechanism of effective components of Angelica dahurica by bioinformatics analysis.Methods The target genes of effective active components of Angelica dahurica were analyzed through traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP).The target genes of melanin related diseases were obtained from the comprehensive database GeneCards,the genes related to melanin deposition disease were screened from the chip GSE114445,and the genes related to melanin disease in the target genes of effective active components of Angelica dahurica were obtained after intersection.Screening autophagy related genes from autophagy database,the genes related to melanin disease and autophagy in the target genes of effective active ingredients of Angelica dahurica were obtained by intersection of the genes related to melanin disease and autophagy in the target genes of effective active ingredients of Angelica dahurica.The gene enrichment pathways related to melanin disease and autophagy in the target genes of effective active components of Angelica dahurica were analyzed online by Metascape.Results Totally 5342 drug target genes of Angelica dahurica were obtained,including 152 genes related to melanin disease,and nine genes related to melanin disease and autophagy.The genes related to melanin disease and autophagy in the target genes of effective active components of Angelica dahurica were mainly enriched in phosphoinositide 3-kinase protein kinase B(PI3K-Akt),mitogen-activated protein kinase(MAPK)and rat sarcoma viral oncogene homolog(RAS)pathways.Conclusion A total of nine Angelica dahurica drug target genes related to melanin disease and autophagy were screened.These nine genes were mainly enriched in PI3K-Akt,MAPK and RAS pathways.The results of this study preliminarily revealed the material basis of the efficacy of Angelica dahurica,which can provide references for clarifying the mechanism of Angelica dahurica promoting melanin degradation.