取材时间和部位对脐带静脉血pH值的影响

Effect of sampling time and site on the pH of umbilical cord venous blood

目的探讨分析脐带血取样时不同取材时间和部位对脐带静脉血pH值的影响。方法随机抽取符合标准的孕妇170例纳入研究。在胎儿分娩后5 min、15 min、30 min采集游离段和连接胎盘段脐带静脉血,测量相应血样pH值。结果随着取材时间的延长,游离段和连接胎盘段的脐静脉血pH值呈逐渐降低趋势;且游离段的脐静脉血pH值显著高于连接胎盘段部位,组间、时点间、组间·时点间交互作用差异有统计学意义(P<0.05)。结论随着脐带血取材时间的延长,脐静脉血pH值逐渐降低,游离段的脐静脉血pH值高于连接胎盘段部位。为保证脐静脉血pH值的测量结果能更好地反映胎儿实际生理情况,应尽快进行脐带血采样,并尽可能在距离胎盘较远的游离段取样。

Objective To investigate the effect of sampling time and site on the pH value of umbilical cord venous blood.Methods A total of 170 pregnant women who met the criteria were randomly selected and included in the study.Free and connected placental cord venous blood were collected at 5 min,15 min and 30 min after fetal delivery,and the pH of the corresponding blood samples was measured.Results With the extension of sampling time,the pH value of the free segment and the connected placenta segment decreased gradually;the pH value of the free segment was significantly higher than that of the connected placenta segment,and there were significant differences in interaction between groups,time points and time points between groups(P<0.05).Conclusion With the extension of sampling time of cord blood,the pH value of umbilical vein blood decreases gradually.The pH value of free segment of umbilical venous blood is higher than that with connecting placental segment.Therefore,in order to ensure that the measurement results of pH value of umbilical venous blood can better reflect the actual physiological situation of the fetus,umbilical cord blood sampling should be conducted as soon as possible,and sampling should be conducted in the free segment far from the placenta.