Box-Behnken响应面法优化竹沥半夏炮制工艺

The Box-Behnken response surface method to optimize the preparation technology of Succus bambusae pinella preparata

目的优化竹沥半夏炮制工艺,为竹沥半夏饮片临方炮制规范化研究提供参考。方法采用Box-Behnken响应面法设计分析,以浸出物、次黄嘌呤、尿苷、腺嘌呤、鸟苷的综合评分为评价指标,考察竹沥半夏辅料用量、炮制时间、干燥温度等3个因素,对竹沥半夏炮制工艺进行优选,同时对最佳工艺进行验证。结果竹沥半夏最佳炮制工艺为:每100 g姜半夏,鲜竹沥15 g,炮制时间1 h,干燥温度44℃。结论该工艺简易可行、竹沥半夏质量稳定可靠,为竹沥半夏的临床应用提供了科学依据。

Objective In order to optimize the preparation technology of Succus bambusae pinella preparata,and so as to provide a reference for the standardized research on the prescription-based processing of Succus bambusae pinella preparata.Methods Using the Box-Behnken response surface method design and analysis,taking the comprehensive scores of extractable,hypoxanthine,uridine,adenine and guanosine as the evaluation index,the three factors of excipient dosage,drying temperature and processing time of Succus bambusae pinella preparata were investigated,and the preparation technology of Succus bambusae pinella preparata was optimized,and the best process was used to verify it.Results The best conditions for the preparation technology of Succus bambusae pinella preparata were 100 g of Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine,the fresh bamboo sap was 15 g,the processing time was 1 h,and the drying temperature was 44℃.Conclusion The preparation technology is simple and feasible,and the quality of Succus bambusae pinella preparata is stable and reliable,which provides a scientific basis for the clinical application of Succus bambusae pinella preparata.