摘要
The biomimetic construction of a microstructural–mechanical–electrical anisotropic microenvironment adaptive to the native cardiac tissue is essential to repair myocardial infarction(MI).Inspired by the 3D anisotropic characteristic of the natural fish swim bladder(FSB),a novel flexible,anisotropic,and conductive hydrogel was developed for tissue-specific adaptation to the anisotropic structural,conductive,and mechanical features of the native cardiac extracellular matrix.The results revealed that the originally stiff,homogeneous FSB film was tailored to a highly flexible anisotropic hydrogel,enabling its potential as a functional engineered cardiac patch(ECP).In vitro and in vivo experiments demonstrated the enhanced electrophysiological activity,maturation,elongation,and orientation of cardiomyocytes(CMs),and marked MI repair performance with reduced CM apoptosis and myocardial fibrosis,thereby promoting cell retention,myogenesis,and vascularization,as well as improving electrical integration.Our findings offer a potential strategy for functional ECP and provides a novel strategy to bionically simulate the complex cardiac repair environment.
出处
《Research》
SCIE
EI
CSCD
2024年第1期489-507,共19页
研究(英文)
基金
the National Natural Science Foundation of China(U21A20173,52003113,31922043,82102228,and 32071363)
Guangdong Basic and Applied Basic Research Foundation(2021A1515010745 and 2020A1515110356)
Medical Research Project of Guangdong Province(A2021462)
Science and Technology Planning Project of Guangdong Province(2020B1212060037)
Key Research&Development Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory(2018GZR110104002)
Shenzhen Fundamental Research Key Project(JCYJ20200109150641992).