大白菜CHS基因鉴定及其在高氮水平下转录表达分析

Identification of CHS gene in Chinese cabbage and transcriptional expression analysis under high nitrogen levels

为了探究高氮水平引起大白菜叶柄黑点症加剧的机制,通过对抗、感叶柄黑点症大白菜品系进行正常氮和高氮水平处理,处理前和处理后不同时间对叶柄取样并进行转录组测序,然后再对大白菜查尔酮合酶(chalcone synthetase,CHS)基因进行鉴定并分析不同的大白菜CHS基因在正常氮水平和高氮水平、抗性品系和感性品系之间的差异表达,结果表明,共鉴定到7个大白菜CHS基因,其中有3个(BrCHS1、BrCHS3及BrCHS4)在高氮水平下表达量比正常氮水平下高,且在高氮水平下感性品系表达量高于抗性品系。因此推测这3个大白菜CHS基因可能与大白菜叶柄黑点症的形成有关。研究结果为揭示大白菜叶柄黑点症发生机制奠定了基础。

In order to investigate the mechanism of exacerbation of Chinese cabbage petiole black spot disease caused by high nitrogen levels,the resistant and suscetible Chinese cabbage were treated with normal and high nitrogen levels,and sampled petioles were sampled at different time before and after treatment for transcriptome sequencing.The Chinese cabbage chalcone synthase(CHS)gene was identified and analyzed for different Chinese cabbage CHS genes at normal and high nitrogen levels.The differential expression between resistant and susceptible Chinese cabbage showed that a total of 7 CHS genes were identified,of which 3 genes(BrCHS1,BrCHS3,and BrCHS4)had higher expression levels under high nitrogen levels than the normal nitrogen levels,and the expression level of susceptible Chinese cabbage was higher than that of resistant Chinese cabbage under high nitrogen levels.Therefore,it is speculated that these three Chinese cabbage CHS genes may be related to the formation of Chinese cabbage petiole black spot disease.This study lays the foundation for revealing the mechanism of black spot disease on the petiole of Chinese cabbage.