金钗石斛组培苗壮苗培养研究

Optimization of tissue culture and rapid propagation system of dendrobium nobile

为研究金钗石斛组培苗快速生长的影响因素,以生长素NAA、水解乳蛋白、MS培养基有机成分、活性炭四因素三水平设置正交试验,观察金钗石斛组培苗壮苗培养的生长表现,从而确定最佳培养基组合。试验结果表明:0.10 g/L活性炭+0.50 mg/L NAA+0.05 g/L水解乳蛋白+1倍有机成分组合对组培苗各生长效果最好;加入NAA可以明显增加组培苗生长的高度和茎粗。本研究可为金钗石斛组培快繁研究奠定基础。

In order to study the factors that affect the rapid growth of Dendrobium nobile tissue culture seedlings,this experiment took Dendrobium nobile tissue culture seedlings as the experimental material,and set up an orthogonal test at the three levels of auxin NAA,hydrolyzed milk protein,organic components of MS medium,and activated carbon to observe the growth performance of Dendrobium nobile tissue culture seedlings,so as to determine the best medium combination.The results showed that the combination of 0.10 g/L activated carbon+0.50 mg/L NAA+0.05 g/L hydrolyzed milk protein+1 fold organic component had the best effect on the growth of tissue culture seedlings;and NAA can significantly increase the growth height and coarseness of tissue culture seedlings.This study lays a foundation for the study of tissue culture and rapid propagation of Dendrobium nobile.