秋水仙素处理对青花菜种子萌发及幼苗生长的影响

Effects of colchicine treatment on seed germination and seedling growth of broccoli

为探究秋水仙素处理对青花菜种子萌发及幼苗生长的影响,本试验以青花菜种子为材料,采用不同质量分数(0.03%、0.05%、0.07%、0.10%、0.20%、0.30%)秋水仙素浸种不同时间(12、24 h),以蒸馏水浸种处理为对照(CK),测定种子萌发指标及幼苗的形态和生理生化指标。结果表明:与CK相比,浸种12 h时,各处理组发芽率、发芽势无显著性差异,0.20%、0.30%秋水仙素处理组的活力指数显著增加;浸种24h时,各处理组各发芽指标均降低;0.10%~0.30%秋水仙素处理使青花菜幼苗鲜质量、茎粗增加,株高、叶长、叶宽降低,抗氧化酶(SOD、CAT)活性及渗透调节物质、硫苷含量升高,而丙二醛含量下降;对青花菜测定指标进行隶属函数分析得出,0.30%秋水仙素浸种12和24 h的平均隶属函数值排名均为第一。综合分析得出,0.30%秋水仙素浸种12 h为促进青花菜种子萌发和幼苗生长的最佳处理组合。

In order to explore the effects of colchicine treatment on the seed germination and seedling growth of broccoli,this experiment took broccoli seeds as materials,soaked with different concentrations of colchicine(0.03%,0.05%,0.07%,0.10%,0.20%,0.30%)for different time(12,24 h),and compared with the seed soaking treatment in distilled water(CK).The effects of colchicine treatment on the seed germination,seedling morphological,physiological and biochemical characteristics of broccoli were studied.The results showed that compared with CK,there was no significant difference in germination rate and germination potential among the treatment groups after soaking for 12 h,and the vitality index of 0.20%and 0.30%colchicine trearment groups increased significantly.After soaking for 24 h,all germination indexes decreased in all treatment groups.0.10%-0.30%colchicine treatment increased the fresh weight and stem thickness of broccoli seedlings,decreased plant height,leaf length and width,increased the antioxidant enzyme(SOD,CAT)activities,osmoregulatory substances and glucosinolates content,while decreased the malondialdehyde content.According to the analysis of membership functions of the measurement indicators of broccoli,the average membership function values of 0.30%colchicine soaking for 12 and 24 h ranked first.In a comprehensive analysis,0.30%colchicine soaking for 12 h was considered as the best treatment combination to promote seed germination and seedling growth of broccoli.