CHAC1在膀胱尿路上皮癌恶性进展中的潜在作用

Potential Role of CHAC1 in Malignant Progression of Bladder Urothelial Carcinoma

目的 探究CHAC1在膀胱尿路上皮癌(BLCA)中的生物学特征及其临床相关性。方法 从肿瘤基因组图谱和人类蛋白质图谱等数据库获取CHAC1的原始数据,应用R软件、MEXPRESS平台和TISIDB平台,对CHAC1在BLCA中的潜在作用进行基因表达分析、生存分析、基因拷贝数变异及甲基化分析、基因富集分析、免疫浸润分析。结果 与正常组织相比,CHAC1在多种肿瘤组织中异常表达(P<0.05),尤其在BLCA中呈现高表达(P<0.001);CHAC1高表达预示着BLCA的不良预后,且与CHAC1低甲基化水平有关;CHAC1富集分析发现其参与了包括氨基酸代谢、GSH代谢等多种代谢相关的生物学过程;CHAC1在BLCA中的表达水平与多种免疫细胞的过度浸润有关,包括树突状细胞,活化的树突状细胞,中性粒细胞,巨噬细胞,调节性T细胞,Th1细胞,Th2细胞,B细胞和NK CD56dim细胞。结论 CHAC1可能成为评估BLCA预后和调节免疫浸润水平的潜在生物标志物和治疗靶点。

Objective To investigate the biological characteristics and clinical relevance of CHAC1 in bladder urothelial carcinoma(BLCA).Methods The original data of CHAC1 was obtained from the Cancer Genome Atlas and the Human Protein Atlas.R software,MEXPRESS platform and TISIDB platform were used for gene expression analysis,survival analysis,copy number variation and methylation analysis,gene enrichment analysis and immune infiltration analysis of CHAC1.Results Compared with normal tissues,CHAC1 is abnormally expressed in a variety of tumor tissues(P<0.05),especially in BLCA(P<0.001).High expression of CHAC1 predicts poor prognosis of BLCA,which is related to CHAC1 hypomethylation level.CHAC1 enrichment analysis showed that it was involved in a variety of metabolism-related biological processes,including amino acid metabolism and GSH metabolism.The expression level of CHAC1 in BLCA was associated with excessive infiltration of a variety of immune cells,including dendritic cells,activated dendritic cells,neutrophils,macrophages,regulatory T cells,Th1 cells,Th2 cells,B cells,and NK CD56dim cells.Conclusion CHAC1 may become a potential biomarker and therapeutic target for evaluating BLCA prognosis and modulating levels of immune infiltration.