血清miR-218-5p和miR-124-3p在阿尔茨海默病中的表达水平及诊断价值

Expression and diagnostic value of serum miR-218-5p and miR-124-3p in Alzheimer's disease

目的 探讨血清miR-218-5p、miR-124-3p在阿尔茨海默病(AD)患者中的表达水平及临床意义。方法 选取2021年5-12月江苏大学附属医院神经内科收治的42例初诊AD患者(AD组),另选取同期23名健康体检者为对照组,采用简易智力状态检查量表(MMSE)、蒙特利尔认知评估量表(MoCA)评分评估AD患者的认知功能,用荧光定量PCR检测血清中miR-218-5p、miR-124-3p的表达水平,分析两者与MMSE、MoCA评分的相关性,并应用受试者工作特征(ROC)曲线及logistic回归模型评价血清miR-218-5p、miR-124-3p在AD中的诊断价值。结果 AD组MMSE、MoCA评分[(16.50±5.22)、(14.50±4.10)分]低于对照组[(28.67±1.12)、(27.61±1.23)分],差异均有统计学意义(t=-14.39、-19.18;P均<0.01)。AD组血清miR-218-5p表达水平[1.91(1.04~3.18)]高于对照组[1.00(1.00~2.00)],血清miR-124-3p表达水平(0.67±0.36)低于对照组(1.08±0.25),差异均有统计学意义(Z=-3.68,t=-5.40;P均<0.01)。相关性分析显示,血清miR-218-5p表达水平与MMSE、MoCA评分均成负相关(r=-0.72、-0.61,P均<0.01);血清miR-124-3p表达水平与MMSE、MoCA评分均成正相关(r=0.42、0.59,P均<0.01)。ROC曲线结果显示,miR-218-5p诊断AD的曲线下面积(AUC)为0.777(95%CI:0.657~0.898),miR-124-3p诊断AD的AUC为0.810(95%CI:0.705~0.914);两者联合诊断AD时,AUC为0.884(95%CI:0.804~0.964),敏感度为76.19%,特异度为86.96%。结论 血清miR-218-5p、miR-124-3p有望成为AD临床辅助诊断的潜在生物学标志物。

Objective To investigate the expression levels and clinical significance of serum miR-218-5p and miR-124-3p in patients with Alzheimer ' s disease(AD).Methods Forty-two patients with AD who admitted to the Department of Neurology of the Affiliated Hospital of Jiangsu University from May to December 2021 were enrolled as the AD group,and twenty-three healthy physical examination people during the same period were selected as the control group.The cognitive function of AD patients was evaluated by mini-mental state examination(MMSE) and Montreal cognitive assessment(MoCA).The expression levels of miR-218-5p and miR-124-3p in serum were detected by real-time fluorescence quantitative PCR,and the correlation between them and the scores of MMSE and MoCA were analyzed.The diagnostic value of serum miR-218-5p and miR-124-3p for AD were evaluated by receiver operating characteristics(ROC)curve and logistic regression model.Results The scores of MMSE and MoCA in AD Group[(16.50±5.22),(14.50±4.10)score]were significantly lower than those in control group[(28.67 ± 1.12),(27.61 ± 1.23)score],the differences were statistically significant(t=-14.39,-19.18;both P<0.01).The expression level of miR-218-5p in AD group[1.91(1.04-3.18)]was higher than that in control group[1.00(1.00-2.00)],and the expression level of miR-124-3p in AD group(0.67±0.36)was lower than that in control group(1.08±0.25),the differences were statistically significant(Z=-3.68,t=-5.40;both P<0.01).Correlation analysis showed that the expression level of serum miR-218-5p was negatively correlated with the scores of MMSE and MoCA(r=-0.72,-0.61;both P<0.01).The expression level of serum miR-124-3p was positively correlated with the scores of MMSE and MoCA(r=0.42,0.59;both P<0.01).ROC curve showed that the area under the curve(AUC)of miR-218-5p for AD diagnosis was 0.777(95% CI:0.657-0.898),the AUC of miR-124-3p for AD diagnosis was 0.810(95%CI:0.705-0.914).When miR-218-5p and miR-124-3p were combined to diagnose AD,the AUC was 0.884(95%CI:0.804-0.964),the sensitivity was 76.19%,and the specificity was 86.96%.Conclusion Serum miR-218-5p and miR-124-3p could be potential biomarkers for the clinical auxiliary diagnosis of AD.