摘要
Protein phosphorylation,catalyzed by protein kinases(PKs),is one of the most prevalent post-translational modifications(PTMs)in eukaryotes.Phosphorylation occurring at different positions in a protein sequence can possess distinct functional impacts,and sitespecific phosphorylation may drastically alter a protein’s conformation,activity,and trafficking.Traditionally,biologists usually focused on studying regulatory mechanisms of phosphorylation in a limited number of proteins,mainly due to a lack of highthroughput technology.During the past two decades,advances in liquid chromatography coupled with tandem mass spectrometry(LC-MS/MC)have boomed a revolutionary technology named phosphoproteomics,which can simultaneously quantify thousands of phosphorylation sites(p-sites),and provide a great opportunity for a systems-level understanding of phosphorylation.Besides LCMS/MC,other approaches such as immunohistochemistry(IHC)and immune-detection by sequencing(ID-seq)have also emerged.
作者
彭迪
贾大
夏宏光
张珞颖
黄鹏宇
薛宇
Di Peng;Da Jia;Hongguang Xia;Luoying Zhang;Pengyu Huang;Yu Xue(Department of Bioinformatics and Systems Biology,MOE Key Laboratory of Molecular Biophysics,Hubei Bioinformatics and Molecular Imaging Key Laboratory,College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China;Key Laboratory of Birth Defects and Related Diseases of Women and Children,Department of Paediatrics,West China Second University Hospital,State Key Laboratory of Biotherapy,Sichuan University,Chengdu 610041,China;Department of Biochemistry&Research Center of Clinical Pharmacy of The First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310058,China;Institute of Biomedical Engineering,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300192,China;Nanjing University Institute of Artificial Intelligence Biomedicine,Nanjing 210031,China)
基金
supported by the National Key R&D Program of China(2022YFC2704300,2021YFF0702000 and 2021ZD0201300)
the National Natural Science Foundation of China(32341020,32341021,and 31930021)
Hubei Innovation Group Project(2021CFA005)
Hubei Province Postdoctoral Outstanding Talent Tracking Support Program
the Research Core Facilities for Life Science(HUST)。