麝香保心丸对自发性高血压大鼠心脏血管新生的影响

Effect of Shexiang Baoxin pill on cardiac angiogenesis in spontaneously hypertensive rats

目的 探讨麝香保心丸对自发性高血压大鼠(SHR)心脏组织血管新生的影响。方法 将20只12周龄雄性SHR随机分为实验组和模型组,另选10只12周龄雄性SD大鼠作为正常组。实验组大鼠每日予以麝香保心丸(45 mg·kg^(-1))灌胃,用无创尾动脉血压测量仪监测血压,每4周1次,8周后取心脏组织。用血小板-内皮细胞黏附分子(PECAM-1/CD31)免疫荧光染色法观察CD31表达情况;用蛋白质印迹法检测心肌血管内皮生长因子(VEGF)、心肌血管内皮生长因子受体2(VEGF-R2)、碱性成纤维细胞生长因子(bFGF)和磷酸化蛋白激酶B(p-Akt)/蛋白激酶B(Akt)蛋白的表达水平。结果 实验组、模型组与同时间点的正常组比较,血压均显著升高(均P<0.01),但同时间点的实验组与模型组比较,血压差异均无统计学意义(均P>0.05)。正常组、模型组、实验组的CD31表达率分别为(3.79±0.84)%、(2.54±0.42)%和(3.56±0.49)%;VEGF水平分别为0.95±0.10、0.73±0.08和0.94±0.15;VEGF-R2水平分别为0.85±0.10、0.61±0.14和0.80±0.10;bFGF水平分别为0.84±0.04、0.51±0.21和0.74±0.14;p-Akt/Akt水平分别为0.85±0.15、0.57±0.13和0.80±0.20。正常组上述指标与模型组比较,实验组上述指标与模型组比较,在统计学上差异均有统计学意义(均P<0.05)。结论 麝香保心丸能够促进自发性高血压大鼠心脏组织中微血管的新生,其机制可能与激活PI3K/Akt磷酸化、上调心肌组织bFGF、VEGF及其受体VEGF-R2有关。

Objective To explore the effect of Shexiang Baoxin pill on cardiac tissue angiogenesis in spontaneously hypertensive rats(SHR).Method Twenty 12 week old male SHR were randomly divided into experimental group and model group,with 12 week old male SD rats as the normal control group.The experimental group rats were orally administered with Shexiang Baoxin pill(45 mg·kg^(-1))daily,and their blood pressure was monitored using a non-invasive tail artery blood pressure gauge every four weeks.Eight weeks later,cardiac tissue was taken for platelet endothelial cell adhesion molecule-1(PECAM-1/CD31)immunofluorescence staining to observe CD31 expression level.Use protein blotting to detect the expression levels of myocardial endothelial growth factor(VEGF),myocardial endothelial growth factor receptor 2(VEGF-R2),basic fibroblast growth factor(bFGF)and phosphorylated protein kinase B(p-Akt)/protein kinase B(Akt)proteins.Result There was significant increase in blood pressure between the experimental group,model group and normal group at the same time point(all P<0.01),but there was no statistically significant difference in blood pressure changes between the experimental group and model group at the same time point(all P>0.05).The CD31 expression rates of the normal group,model group and experimental group were(3.79±0.84)%,(2.54±0.42)% and(3.56±0.49)%;VEGF levels were 0.95±0.10,0.73±0.08 and 0.94±0.15;VEGF-R2 levels were 0.85±0.10,0.61±0.14 and 0.80±0.10;bFGF levels were 0.84±0.04,0.51±0.21 and 0.74±0.14;p-Akt/Akt levels were 0.85±0.15,0.57±0.13 and 0.80±0.20,respectively.The differences between the normal group and the model group,as well as the experimental group and the model group,were statistically significant(all P<0.05).Conclusion Shexiang Baoxin pill can promote the neovascularization of microvessels in the heart tissue of spontaneously hypertensive rats,and its mechanism may be related to the activation of PI3K/Akt phosphorylation,upregulation of bFGF,VEGF and their receptor VEGF-R2 in myocardial tissue.