济川煎对慢传输型便秘大鼠肠道功能的影响及作用机制的研究

Effect and mechanism of Jichuan decoction on intestinal function in rats model of slow transit constipation

目的 探讨中药“济川煎”对于慢传输型便秘(STC)大鼠排便情况的影响。方法 将36只SD大鼠随机分为模型组、实验组和对照组,除对照组外,其余2组均用2%大黄酸混悬液灌胃法建立STC大鼠模型,实验组用170%济川煎浓缩液10 mL·kg^(-1)灌胃,对照组和模型组用0.9%NaCl灌胃,每天灌胃1次,连续30 d。通过粪便称质量、记录首次粪便排出时间计算粪便干湿质量比及排便时间,用试剂盒检测血浆中P物质(SP)及血管活性肽(VIP)含量,用实时荧光定量聚合酶链反应(qRT-PCR)技术检测肠道菌群变化。结果 对照组、模型组和实验组粪便干湿质量比分别为(44.86±4.40)%、(53.44±3.52)%和(44.28±4.33)%,排便时间分别为(340.17±34.91)、(424.67±37.26)和(377.36±38.87)min,血浆中SP分别为(79.86±7.56)、(60.30±2.41)和(78.84±5.83)pg·mL^(-1),血浆中VIP分别为(30.56±2.87)、(23.38±6.62)和(28.39±2.37)pg·mL^(-1),双歧杆菌分别为7.77±0.04、5.19±0.09和7.45±0.03;乳酸杆菌数分别为6.40±0.07、4.10±0.05和6.36±0.08。模型组以上指标与对照组比较,实验组以上指标与模型组比较,在统计学上差异均有统计学意义(P<0.05,P<0.01)。结论 中药“济川煎”能够改善慢传输型便秘大鼠的排便情况。

Objective To investigate the effect of Jichuan decoction on defecation in rats with slow transit constipation(STC).Methods A total of 36 SD rats were divided into model group,experimental group and control group.Except the control group,STC rat models were established in the other two groups by gavage of 2%rhein acid suspension.The experimental group was given 170%Jichuan decoction of 10 mL·kg^(-1)by gavage,while the control group and model group were given with 0.9%NaCl by gavage,once a day for 30 days.Fecal dry-wet weight ratio and colon transport time were observed by fecal weighing and recording of first fecal discharge time;substance P(SP)and vasoactive peptide(VIP)contents in plasma were detected by kit;changes in intestinal flora were detected by real-time fluorescent quantitative polymerase chain reaction(qRT-PCR).Results The dry-wet ratio of feces in control group,model group and experimental group were(44.86±4.<40)%,(53.44±3.52)%and(44.28±4.33)%,respectively;the colon transport time were(340.17±34.91),(424.67±37.26)and(377.36±38.87)min,respectively;plasma SP were(79.86±7.56),(60.30±2.41)and(78.84±5.83)pg·mL^(-1),respectively;plasma VIP were(30.56±2.87),(23.38±6.62)and(28.39±2.37)pg·mL^(-1),respectively;the numbers of Bifidobacterium were 7.77±0.04,5.19±0.09 and 7.45±0.03,respectively;the numbers of Lactobacillus were6.40±0.07,4.10±0.05 and 6.36±0.08,respectively.There were statistically significant differences in the above indexes between model group and control group,and between experimental group and model group(P<0.01,P<0.05).Conclusion Jichuan decoction can improve the defecation of rats with slow transit constipation.