合成大麻素免疫胶体金现场快速筛查方法的评价

Evaluation of a rapid field screening method for synthetic cannabinoid immunocolloidal gold

本研究以新精神活性物质合成大麻素为主要研究对象,拟对五类共计13种合成大麻素的免疫胶体金(碳)快速筛查方法进行应用研究。采用标准样本和尿液添加样本对方法的灵敏度进行测定,确证免疫胶体金(碳)技术的阈值,同时测定检测结果的稳定性;采用21种常见毒品和试剂盒检测范围外的7种合成大麻素样本对方法特异性进行验证;采用经LC/MS检测的58份缴获样本进行准确性验证,并应用于嫌疑吸毒者尿液检测。结果表明,该技术适用于缴获可疑样本的现场快速筛查,适用于部分目标物检测限低的嫌疑吸毒者尿液样本的筛查。

This study takes synthetic cannabinoids,a new psychoactive substance,as the main object of study,and intends to study the application of immunocolloidal gold(carbon)rapid screening methods for 13 kinds of synthetic cannabinoids in five categories.The sensitivity of the method was determined by using standard samples and urine addition samples,to verify/determin the threshold of the immunocolloidal gold(carbon)technique,and to determine the stability of the detection results.The specificity of the method was verified by using 21 common drugs and 7 synthetic cannabinoid samples outside the detection range of the kit.The accuracy of 58 seized samples and 34 suspected drug user urine samples tested by LC/MS were verified.The results show that the technique is capable for rapid screening of seized suspect samples and the targetswith low limits of detection from urine samples of suspected drug user.