气阴两虚夹瘀型2型糖尿病患者外周血LncRNA TUG 1与血管病变标志因子的相关性

Correlation between Long Non-Coding RNA Taurine Regulatory Gene 1 and Marker Factors of Vascular Disease in Type 2 Diabetic Patients with Qi-Yin Deficiency and Blood Stasis Syndrome

目的分析气阴两虚夹瘀型2型糖尿病患者外周血长链非编码RNA牛磺酸调节基因1(long non-coding RNA taurine regulatory gene 1,LncRNA TUG1)与血管病变因子的相关性。方法随机选取该院内分泌科住院的气阴两虚夹瘀型2型糖尿病患者50例作为观察组,另选取46例健康体检者作为健康对照组。检测餐后2 h血糖(2-hour postprandial blood glucose,2 h PG)、空腹血糖(fasting plasma glucose,FPG)、糖化血红蛋白(glycosylated hemoglobin,HbA1c)、内皮素(endothelin,ET)、一氧化氮(nitric oxide,NO)水平及LncRNA TUG1表达;经Pearson线性相关分析,血清LncRNA TUG1与ET、NO、HbA1c、血糖指标的相关性。结果与健康对照组比较,气阴两虚夹瘀型2型糖尿病患者LncRNA TUG1、HbA1c、FPG、2 h PG、ET水平明显升高(P<0.01),NO水平明显降低(P<0.01),差异具有统计学意义。Pearson相关性分析显示,LncRNA TUG1与NO水平呈显著负相关(P<0.01);与ET、HbA1c、FPG、2 h PG水平呈显著正相关(P<0.01)。结论气阴两虚夹瘀型2型糖尿病患者外周血LncRNA TUG1高表达,与HbA1c、ET、血糖呈正相关,与NO水平呈负相关,与血管病变标志因子关系密切,存在一定的相关性,可作为潜在预测指标。

Objective To analyze the correlation between long non-coding RNA taurineregulatory gene 1(LncRNA TUG1)and vascular disease factors in peripheral blood of type 2 diabetes mellitus patients with Qi-Yin deficiency and blood stasis syndrome.Methods Fifty patients with type 2 diabetes mellitus with Qi-Yin deficiency and blood stasis syndrome in the Department of Endocrinology of the hospital were randomly selected as the observation group and 46 healthy people were selected as the healthy control group.The levels of 2-hour postprandial blood glucose(2 h PG),fasting plasma glucose(FPG),glycosylated hemoglobin(HbA1c),endothelin(ET),nitric oxide(NO)and the expression of LncRNA TUG1 were detected.Pearson linear correlation analysis was performed to analyze the correlation between serum LncRNA TUG1 and ET,NO,HbA1c and blood glucose indexes.Results Compared with those of the healthy control group,the levels of LncRNA TUG1,HbA1c,FPG,2 h PG and ET in the observation group were significantly increased(P<0.01),the level of NO decreased significantly(P<0.01),and the difference was statistically significant.Pearson correlation analysis showed that LncRNA TUG1 was negatively correlated with the level of NO(P<0.01).It was positively correlated with ET,HbA1c,FPG and 2 h PG(P<0.01).Conclusion The expression of LncRNA TUG1 in peripheral blood of patients with type 2 diabetes mellitus with Qi-Yin deficiency and blood stasis syndrome is positively correlated with HbA1c,ET and blood glucose,and negatively correlated with NO level.It is closely related to the marker factors of vascular disease,and can be used as a potential predictor.