数据挖掘与网络药理探讨花类药治痤疮的规律机制

Discussion on the Regularity and Mechanism of Flower Drugs in Treating Acne by Data Mining and Network Pharmacology

目的探讨滇南刘复兴学派用花类药治痤疮的规律及高频花类药的机制。方法收集门诊医案,进行性味归经、类别功效和关联规则分析;筛选高频花类药及痤疮靶点,取交集基因;构建中药-成分-靶点网络及PPI网络,进行GO及KEGG分析,可视化分子对接结果。结果以清热药为主,核心药物11味;高频花类药为金银花、野菊花、红花、玫瑰花,有6个核心成分和7个核心靶点,涉及癌症、脂质、内分泌等通路,分子对接结合较好。结论滇南刘复兴学派注重中上二焦、气血同调,以清热为大法、五味消毒饮为底方,力求攻补兼施、寒热平调、阴阳互滋;花类药可能通过MAPK通路、免疫-炎症反应、脂质、内分泌等作用于痤疮靶点,其中豆甾醇、β-谷甾醇可能是主要成分。

Objective To explore the rule of treating acne with flowers and the mechanism of high-frequency flowers by Liu Fuxing School in southern Yunnan.Methods Collect outpatient medical records and analyze the tropism of sex and taste,category efficacy and association rules;Screening high-frequency flowers and acne targets,and taking intersection genes;Traditional Chinese medicine-component-target network and PPI network were constructed,and GO and KEGG analysis were carried out to visualize the molecular docking results.Results Heat-clearing drugs were the main drugs,with 11 core drugs.High-frequency flower drugs are honeysuckle,chrysanthemum indicum,safflower and rose,which have 6 core components and 7 core targets,involving cancer,lipid,endocrine and other pathways,and have good molecular docking and binding ability.Conclusion Liu Fuxing School in southern Yunnan pays attention to the middle and upper energizer,harmonizing qi and blood,taking clearing away heat as the main method and five-flavored disinfectant as the basic prescription,and strives for both attack and tonic,balancing cold and heat,and nourishing yin and yang.Flower drugs may act on acne targets through MAPK pathway,immune-inflammatory reaction,lipid and endocrine,among which stigmasterol andβ-sitosterol may be the main components.