钙果复合果浆的功能成分及其减肥降脂作用

Functional Composition of Calcium Fruit Composite Pulp Product and the Effects ofLosing Weight and Lowering Lipid

目的:枸杞、沙棘和钙果是我国北方特有的食药同源食物,为了深层次开发3种果实的营养成分,以钙果原浆(纯度100%)、沙棘原浆(纯度100%)、枸杞原浆(纯度100%)3种原料,按照体积比2∶1.5∶1调配混合,制成一款酸甜可口的钙果复合果浆,探究其功能成分及减肥作用。方法:采用电感耦合等离子体质谱法测定复合果浆的微量元素,采用高相液相色谱法测定维生素和类胡萝卜素,液相色谱-质谱联用法测定多酚类化合物和有机酸类化合物。建立肥胖小鼠模型,探究复合果浆对肥胖小鼠的减肥作用。结果表明,复合果浆富含多种功能成分,其中,钙含量为(73.671±4.918)μg/L,维生素B_(2)含量为(503.333±2.054)ng/mg,芦丁含量为(8.345±0.091)μg/mL。与模型组相比,剂量组经复合果浆干预后,小鼠体质量明显下降,甘油三酯含量显著降低(P<0.05)。结论:复合果浆对肥胖小鼠具有显著改善作用,本研究为钙果复合果浆的营养保健作用提供了数据支撑,并为预防肥胖提供了一种新的营养干预方法。

Objective:Lycium barbarum,Hippophae rhamnoides L.and calcium fruit are unique food and medicine food in the north of China,in order to develop the nutrients of three kinds of fruits at a deep level,calcium fruit pulp(purity 100%),sea buckthorn pulp(purity 100%),wolfberry pulp(purity 100%)three kinds of raw materials in accordance with the volume ratio of 2∶1.5∶1 mixing and blending to make a sweet and sour calcium fruit composite fruit syrup,to explore its functional composition and weight loss.Methods:Inductively coupled plasma mass spectrometry(ICP-MS)was used to determine the trace elements of the composite fruit pulp,high-phase liquid chromatography(HPLC)was used to determine the vitamins and carotenoids,and liquid chromatography-mass spectrometry(LC-MS)was used to determine the polyphenolic compounds and organic acid compounds.An obese mouse model was established to investigate the weight loss effect of the composite fruit pulp on obese mice.The results showed that the composite fruit pulp was rich in a variety of functional components,including(73.671±4.918)μg/L of calcium,(503.333±2.054)ng/mg of vitamin B_(2),and(8.345±0.091)μg/mL of rutin.Compared with the model group,the dose group showed a significant decrease in the body mass of the mice and a significant decrease in the triglyceride content after the intervention of the composite fruit pulp.content was significantly reduced(P<0.05).Conclusion:Compound fruit pulp has a significant improvement effect on obese mice,and this study provides data support for the nutritional and health care effects of calcium fruit compound fruit pulp,and provides a new nutritional intervention method for obesity prevention.