LINC01138通过靶向miR-559调控结直肠癌SW620细胞增殖、迁移及侵袭的机制研究

LINC01138 Regulates Proliferation,Migration,and Invasion of SW620 Colorectal Cancer Cells through Targeting miR-559

目的探讨LINC01138通过靶向miR-559调控结直肠癌SW620细胞增殖、迁移及侵袭的机制研究。方法本研究采用RT-qPCR分别检测人正常结直肠黏膜细胞FHC、结直肠癌细胞SW620中LINC01138与miR-559的表达,将体外培养的SW620随机分为Blank组、si-NC组、si-LINC01138组、si-LINC01138+si-miR-559组、mimic NC组、miR-559 mimic组、LINC01138 mimic+miR-559 mimic组,CCK-8、划痕实验、Transwell用于检测细胞增殖、迁移和侵袭。LINC01138和miR-559之间的相互作用使用双荧光素酶实验进行验证。结果与FHC相比,在SW620细胞中LINC01138的表达水平上升,miR-559的表达水平下降。抑制LINC01138或上调miR-559可以减弱SW620细胞的增殖、迁移和侵袭能力。结论本研究验证了LINC01138与miR-559在结直肠癌细胞中的相互作用,发现LINC01138通过靶向miR-559来调控结直肠癌细胞的增殖、迁移和侵袭,这些结果可为结直肠癌的治疗提供了新的靶点和策略。

Objective To investigate the effect of LINC01138 on the proliferation,migration,and invasion of SW620 colorectal cancer cells and its mechanism.Methods RT-qPCR was used to detect the expression levels of LINC01138 and miR-559 in the human normal colonic mucosal cells(FHC)and the colorectal cancer cells(SW620).SW620 cells cultured in vitro were randomly divided into 6 groups:si-NC,si-LINC01138,si-LINC01138+si-miR-559,mimic NC,miR-559 mimic and LINC01138 mimic+miR-559 mimic.CCK-8 assay,wound-healing assay,and transwell assay were performed to evaluate cell proliferation,migration,and invasion,respectively.The interaction between LINC01138 and miR-559 was validated using a dual-luciferase reporter assay.Results The expression level of LINC01138 was upregulated,while the expression level of miR-559 was downregulated in the SW620 cells when compared to those in the FHC.Inhibition of LINC01138 or upregulation of miR-559 attenuated the proliferation,migration,and invasion abilities of SW620 cells.Conclusion This study confirms the interaction between LINC01138 and miR-559 in colorectal cancer cells and reveals that LINC01138 regulates the proliferation,migration,and invasion of colorectal cancer cells through targeting miR-559.These findings provide new targets and strategies for the treatment of colorectal cancer.