基于转录组的茎用莴苣抽薹相关差异表达基因分析

Analysis of differentially expressed genes related to bolting in stem lettuce based on transcriptome data

为探究影响茎用莴苣抽薹的相关关键基因,采用高通量转录组测序的方法,研究茎用莴苣产品器官收获期和抽薹期的差异表达基因。结果表明,茎用莴苣种质资源1号在产品器官收获期和抽薹期的差异表达基因数量为6 754个,茎用莴苣种质资源3号在产品器官收获期和抽薹期的差异表达基因数量为5 444个。基因本体(GO)功能富集分析结果表明,差异表达基因主要富集在结合、催化活性、细胞过程、代谢过程、细胞解剖实体等GO条目中。京都基因与基因组百科全书(KEGG)通路富集分析结果表明,茎用莴苣种质资源1号和茎用莴苣种质资源3号分别有133条和129条KEGG代谢通路,其中有128条代谢通路在2份种质资源中均被注释;差异表达基因富集较多的KEGG代谢通路有次生代谢物的生物合成、植物激素信号转导、植物-病原体的相互作用等。转录因子分析结果表明,茎用莴苣产品器官收获期和抽薹期的差异表达基因大多数属于AP2/ERF、bHLH、bZIP、C2H2、MYB、NAC、WRKY等转录因子家族。对茎用莴苣产品器官收获期和抽薹期差异表达基因的研究结果进行综合分析,发现在AP2/ERF、WRKY、bHLH等转录因子家族以及次生代谢物的生物合成、植物激素信号转导等通路中存在较多的差异表达基因,推测这些转录因子家族和代谢通路可能参与茎用莴苣抽薹的调控网络。本研究结果为解析茎用莴苣抽薹相关基因及其分子机制奠定了基础。

To explore the key genes influencing bolting in stem lettuce,high-throughput transcriptome sequencing was employed to study the differentially expressed genes during the organ harvesting and bolting periods of stem lettuce.The results revealed that there were 6754 differentially expressed genes in stem lettuce germplasm resource No.1 during the organ harvesting period and bolting period,while stem lettuce germplasm resource No.3 had 5444 differentially expressed genes during these periods.Gene ontology(GO)functional enrichment analysis indicated that differentially expressed genes were mainly enriched in GO entries such as binding,catalytic activity,cellular processes,metabolic processes,and cellular anatomical entities.Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis showed that stem lettuce germplasm resource No.1 and stem lettuce germplasm resource No.3 had 133 and 129 KEGG metabolic pathways respectively,with 128 metabolic pathways annotated in both germplasm resources.KEGG metabolic pathways with a relatively higher enrichment of differentially expressed genes included biosynthesis of secondary metabolites,plant hormone signal transduction,and plant-pathogen interactions.Transcription factor analysis revealed that most of the differentially expressed genes during the organ harvesting and bolting periods of stem lettuce belonged to transcription factor families such as AP2/ERF,bHLH,bZIP,C2H2,MYB,NAC and WRKY.Comprehensive analysis results indicated that there were numerous differentially expressed genes in transcription factor families such as AP2/ERF,WRKY,bHLH,as well as pathways involving biosynthesis of secondary metabolites and plant hormone signal transduction.It was speculated that these transcription factor families and metabolic pathways could participate in the regulatory network of bolting in stem lettuce.These results can provide scientific basis for understanding the genes related to bolting in stem lettuce and their molecular mechanisms.