柴胡疏肝散调控Th17/Treg平衡对乙型病毒性肝炎小鼠肝功能的影响及其机制

Effect and mechanism of Chaihu Shugan powder regulating Th17/Treg balance on liver function in mice with hepatitis B

目的:探究柴胡疏肝散(CHSGS)调控Th17/Treg平衡对乙型病毒性肝炎(简称乙肝)小鼠肝功能的影响和机制。方法:将小鼠分为对照组、乙型肝炎病毒(HBV)组和HBV+CHSGS组,每组15只。除对照组外,其他两组均采用HBV(1×106mL, 30 mL·kg^(-1))感染以构建乙肝模型。建模成功后,HBV+CHSGS组通过CHSGS灌胃(30 g·mL^(-1),30 mL·kg^(-1))干预,对照组和HBV组灌胃等量生理盐水。6周后检测小鼠肝功能、肝脏单个核细胞中Th17和Treg变化以及RORγ和FOXP3蛋白的表达。结果:与对照组比较,HBV组的谷丙转氨酶(ALT)[(85.24±17.03)U·L^(-1)]、天门冬氨酸氨基转移酶(AST)[(315.39±37.61)U·L^(-1)]、肝脏单个核细胞中Th17(5.22%±0.51%)、Treg(9.96%±0.95%)、Th17/Treg值(0.52±0.07)、RORγ和FOXP3蛋白均升高(P<0.05)。HBV+CHSGS组的ALT[(60.75±8.46)U·L^(-1)]、AST [(201.44±21.87)U·L^(-1)]、肝脏单个核细胞中Th17(3.67%±0.37%)、Th17/Treg值(0.26±0.04)和RORγ蛋白低于HBV组,而Treg(14.30%±1.13%)和FOXP3蛋白高于HBV组(P<0.05)。结论:CHSGS抑制Th17、促进Treg分化,改善HBV感染引起的Th17/Treg比例异常,从而调控免疫炎症反应保护乙肝小鼠的肝功能。

Objective:To explore the effect and mechanism of Chaihu Shugan powder(CHSGS)regulating Th17/Treg balance on liver function in mice with hepatitis B.Methods:We divided mice into control,hepatitis B virus(HBV)and HBV+CHSGS group.Each group has 15 mice.The hepatitis B model was established by HBV(1×10^(6) mL,30 mL·kg^(-1))infection.CHSGS(30 g·mL^(-1),30 mL·kg^(-1))was intervened by intragastric administration.After 6 weeks,liver function,Th17 and Treg changes in liver mononuclear cells,and the expression of RORγand FOXP3 proteins were detected.Results:Compared with the control group,alanine aminotransferase(ALT)[(85.24±17.03)U·L-1],aspartate aminotransferase(AST)[(315.39±37.61)U·L-1],Th17(5.22%±0.51%),Treg(9.96%±0.95%),Th17/Treg ratio(0.52±0.07),RORγand FOXP3 proteins were all increased(P<0.05).ALT[(60.75±8.46)U·L-1],AST[(201.44±21.87)U·L^(-1)],Th17(3.67%±0.37%),Th17/Treg ratio(0.26±0.04)and RORγprotein in HBV+CHSGS group were lower than those in the HBV group,while Treg(14.30%±1.13%)and FOXP3 proteins were higher than those in the HBV group(P<0.05).Conclusion:CHSGS can inhibit Th17 and promote the differentiation of Treg,so as to improve the abnormal Th17/Treg ratio caused by HBV,and regulate the immune inflammatory response to protect liver function of mice with HBV.