割手密SnRK2基因家族全基因组鉴定及干旱胁迫表达分析

Genome-wide identification and expression analysis of the SnRK2 gene family under drought stress in Saccharum spontaneum L.

【目的】探究割手密SnRK2基因家族成员在干旱胁迫中的调控机制,为抗旱性甘蔗品种的选育提供侯选基因。【方法】以全基因组数据为基础从割手密中鉴定SnRK2基因,并对其进行生物信息学分析和干旱胁迫下的表达分析。【结果】在割手密基因组中共鉴定出11个SnRK2基因家族成员,命名为SsSnRK2.1-SsSnRK2.11,且这些基因不均匀地分布于8条染色体上。SnRK2蛋白的氨基酸残基数为227~580,分子质量为25683.53~64695.8 kD,等电点为4.62~8.94,且均为亲水性蛋白。系统发育树可将其分为3个亚组,且同亚组中的保守基序基本相似,外显子数量以7~9个为主。SsSnRK2基因家族成员的启动子中含有多种激素类和逆境胁迫响应类的作用元件。割手密SsSnRK2基因家族成员的表达具有组织特异性。所有的SsSnRK2基因均能不同程度地响应干旱胁迫。【结论】割手密SnRK2基因家族在响应干旱胁迫过程中发挥重要作用,可为割手密的抗逆性研究提供一定的理论依据。

[Objective]This study aims to explore the regulatory mechanism of SnRK2 family members under drought stress in Saccharum spontaneum L.,in order to provide candidate genes for cultivating drought resistant sugarcane varieties.[Methods]This study identified SnRK2 genes in S.spontaneum L.based on whole genome data,and conducted bioinformatics analysis and expression analysis under drought stress.[Results]11 SnRK2 members were identified and named as SsSnRK2.1 to SsSnRK2.11,which were unevenly distributed on 8 chromosomes.The amino acid residues of SsSnRK2s were ranged from 227 to 580,the molecular weights were from 25683.53 kD to 64695.8 kD,the isoelectric points were from 4.62 to 8.94,and all were hydrophilic proteins.The 11 SsSnRK2s could be divided into 3 subgroups,and the conserved motif sequences within the same subgroup were highly similar,with the number of exons ranging from 7 to 9.The promoters of SsSnRK2 members contained various hormone-related and abiotic stress responsive elements.SsSnRK2 members were expressed in a tissue-specific manner.All SsSnRK2 genes were able to respond to drought stress to various degrees.[Conclusion]The SsSnRK2 family plays a significant role in response to drought stress,providing a basis for the study of stress resistance in S.spontaneum L..