Box-Behnken响应面优化超声波辅助双水相提取枇杷叶多糖及其抗氧化活性研究

Ultrasonic-assisted aqueous two-phase extraction of polysaccharide from loquat leaves by Box-Behnken response surface optimization and its antioxidant activity

试验应用Box-Behnken设计响应面法优化超声波辅助双水相提取枇杷叶多糖的工艺条件,探究超声时间、超声功率、超声温度、液料比对枇杷叶多糖提取率的影响,进一步探究枇杷叶多糖的抗氧化活性。结果表明,超声波辅助双水相法提取枇杷叶多糖的最佳工艺条件为超声时间60 min、超声功率180 W、超声温度60℃、液料比70 mL/g,枇杷叶多糖得率达到4.222%。抗氧化活性试验表明,当枇杷叶多糖浓度为1.2 g/L时对1, 1-二苯基-2-三硝基苯肼(DPPH)自由基清除率为73.90%,对羟基自由基清除率为58.45%。研究表明,枇杷叶多糖的提取工艺环保、可行,且枇杷叶多糖具有较好的抗氧化活性。

Box-Behnken design response surface methodology was used to optimize the ultrasonic-assisted aqueous twophase extraction of loquat leaves polysaccharide.The effects of ultrasonic time,ultrasonic power,ultrasonic temperature,and liquid-material ratio on the extraction rate of loquat leaves polysaccharide and the antioxidant activity of loquat leaves polysaccharide were studied.The results showed that the optimum conditions for extracting polysaccharide from loquat leaves by ultrasonic-assisted aqueous two-phase method were ultrasonic time 60 min,ultrasonic power 180 W,ultrasonic temperature 60℃,liquid-material ratio 70 mL/g,and the yield of polysaccharide from loquat leaves reached 4.222%.The antioxidant activity test showed that the DPPH radical scavenging rate was 73.90%and the hydroxyl radical scavenging rate was 58.45%when the concentration of loquat leaves polysaccharide was 1.2 g/L.The study indicates that the extraction technology of loquat leaves polysaccharide is environmentally friendly and feasible,and loquat leaves polysaccharide have good antioxidant activity.