2022年度宿州市德国小蠊对6种常用杀虫剂的抗药性调查

Investigation on the resistance of Blattella germanica to 6 insecticides in Suzhou City in 2022

目的了解宿州市建成区德国小蠊对常用卫生杀虫剂的抗药性情况,为科学开展城区蜚蠊防治提供数据支撑。方法2022年4—6月,在宿州市埇桥区中小餐饮单位、宾馆及农贸集市等环境采捕德国小蠊野生种群,用药膜法对常用6种杀虫剂进行生物学抗药性测定。采用Excel 2024和SPSS 230软件处理和分析检测数据。结果宿州市埇桥区德国小蠊对胺菊酯、高效氯氰菊酯、氯菊酯、顺式氯氰菊酯、溴氰菊酯及敌敌畏6种杀虫剂的24 h死亡率分别为4600%、10000%、9398%、9286%、9495%和9250%,半数击倒时间KT50分别为4492、1821、2485、1941、2051、3205min。依据死亡率抗性判断标准,宿州市德国小蠊对胺菊酯属抗性种群,对氯菊酯、顺式氯氰菊酯、溴氰菊酯及敌敌畏属可能抗性种群,对高效氯氰菊酯属敏感种群。依据击倒抗药性判定标准,宿州市埇桥区德国小蠊野生种群对高效氯氰菊酯产生了低水平抗性(443倍);对顺式氯氰菊酯、氯菊酯、溴氰菊酯和敌敌畏为中等水平抗药性,抗性倍数依次为523倍、538倍、525倍和830倍;对胺菊酯为高水平抗药性(1294倍)。结论宿州市埇桥区德国小蠊野生种群对常用杀虫剂产生了不同程度的抗药性,在防治工作中,应加强抗药性监测,利用监测数据指导杀虫剂的选择和使用,以延缓其抗药性的发展。

Objective To investigate the resistance of Blattella germanica to 6 common insecticides in built-up area of Suzhou City,and provide data support for scientific application of insecticide against cockroach.Methods The wild population of B.germanica was collected in the small and medium-sized restaurants,hotels and agricultural markets in the Yongqiao district of Suzhou City from April to June 2022,and the biological resistance of 6 commonly used insecticides was determined by drug film method.Excel 2024 and SPSS 230 software were used to process and analyze the test data Results The mortality rates of B.germanicain 24 hours against 6 insecticides,tetramethrin,beta-cypermethrin,permethrin,alphamethrin,deltamethrin and dichlorvos were 4600%、10000%、9398%、9286%、9495%and 9250%,respectively.The median knockdown time KT50 was 4492,1821,2485,1941,2051and 3205 min,respectively.According to the mortality resistance criteria,B.germanica was resistant to tetramethrin,possibly resistant to alphamethrin,permethrin,deltamethrin and dichlorvos,and sensitive to beta-cypermethrin.According to the knockdown resistance standard,low resistance to beta-cypermethrin(443 times)and moderate resistance to permethrin,alphamethrin,deltamethrin and dichlorvos(538,523,525 and 830 times,respectively)were developed.Highly resistant to tetramethrin was developed(1294 times)Conclusion B.germanica in Suzhou City developed different degrees of resistance to commonly used insecticides,and high efficiency cypermethrin can still be used moderately,and amine permethrin and other insecticides should be stopped.