鼠李糖乳酪杆菌Glory LG12对克林霉素诱导小鼠肠道菌群紊乱的影响

Effect of Lacticaseibacillus rhamnosus Glory LG12 on Clindamycin-Induced Intestinal Flora Disorders in Mice

为探讨鼠李糖乳酪杆菌Glory LG12对小鼠肠道菌群的影响,将60只BALB/c小鼠适应性喂养1周后,随机分成对照组、模型组、低剂量组、中剂量组和高剂量组。其中对照组小鼠灌胃等体积的生理盐水,模型组灌胃克林霉素和同体积生理盐水,受试组分别按照1.5×10^(6)、1.5×10^(7)、1.5×10^(8)CFU/只灌胃鼠李糖乳酪杆菌Glory LG12。灌胃14 d后,测定小鼠体质量、肠道菌群结构、组织病理变化、肠道屏障功能、肠道通透性和短链脂肪酸浓度。结果表明,与对照组相比,鼠李糖乳酪杆菌Glory LG12使小鼠粪便中乳杆菌、双歧杆菌等有益菌的数量显著提高,产气荚膜梭菌的数量显著下降,并且短链脂肪酸的浓度明显提高。鼠李糖乳酪杆菌Glory LG12对调节小鼠肠道菌群具有显著的改善作用。

To investigate the effect of Lacticaseibacillus rhamnosus Glory LG12 on the intestinal flora of mice,60 BALB/c mice were acclimatized and fed for 1 week,and then randomly divided into control,model,low-dose,medium-dose,and high-dose groups.The control group was gavaged with an equal volume of saline,while the model group was gavaged with clindamycin and the same volume of saline.The test groups were gavaged with Lacticaseibacillus rhamnosus Glory LG12 at doses of 1.5×10^(6),1.5×10^(7),and 1.5×10^(8) CFU/mouse,respectively.After 14 d of gavage,determination of body mass,intestinal flora structure,histopathologic changes,intestinal barrier function,intestinal permeability,and short-chain fatty acid concentration in mice.The results showed that compared with the control group,Lacticaseibacillus rhamnosus Glory LG12 significantly increased the number of beneficial bacteria such as Lacticaseibacillus and Bifidobacterium,significantly decreased the number of Clostridium perfringens,and significantly increased in the concentration of short-chain fatty acids in the feces of the mice.Lacticaseibacillus rhamnosus Glory LG12 demonstrated a significant improvement in regulating the intestinal flora of mice.