杏鲍菇发酵苦荞提取物对小鼠酒精性肝、胃损伤的保护作用

Protective Effect of Tartaty Buckwheat Extract Fermented with Pleurotus eryngii on Alcoholic Liver and Stomach Injury in Mice

目的:研究杏鲍菇发酵苦荞提取物的体外抗氧化活性及体内对酒精性肝脏、胃黏膜损伤的保护作用。方法:本实验检测杏鲍菇发酵苦荞提取物中功能成分的含量,并分析提取物的抗氧化能力;以Lieber-DeCarli酒精液体饲料建立小鼠慢性酒精性肝脏、胃黏膜损伤模型,考察发酵后的苦荞提取物在低、高剂量(1.5 g/kg B.W.、3.0 g/kg B.W.)对肝脏和胃粘膜损伤的保护作用。结果:杏鲍菇发酵苦荞提取液中含有较多的抗氧化成分,其中多酚、黄酮、三萜含量分别为11.40±0.32 mg GAE/g DW、17.19±0.30 mg RE/g DW、7.59±0.24 mg/g,黄酮类物质:芦丁和槲皮素含量分别为13.55±0.05、0.665±0.01 mg/g;杏鲍菇发酵苦荞提取液的铁离子还原抗氧化能力及其对DPPH和ABTS+自由基清除率分别为:16.66±0.65、33.49±1.26、15.68±1.17μmol Trolox/g DW;与模型组相比,高、低剂量组均能显著降低丙二醛(P<0.05)、天冬氨酸氨基转移酶(P<0.01)、丙氨酸氨基转移酶(P<0.01)、乳酸脱氢酶(P<0.05)、白细胞介素1β(P<0.05)水平,并显著提高超氧化物歧化酶(P<0.01)、谷胱甘肽过氧化物酶(P<0.01)水平,下调了活性氧(P<0.01)、鼠肉瘤蛋白(P<0.01)、丝氨酸/苏氨酸激酶(P<0.01)、细胞外信号调节激酶(P<0.05)、丝裂原活化蛋白激酶激酶(P<0.05)的蛋白表达量。结论:杏鲍菇发酵苦荞提取物具有较好的抗氧化活性,并对小鼠慢性酒精性肝脏、胃黏膜损伤具有明显的保护作用。

Objective:To investigate the antioxidant activity of fermented tartaty buckwheat extract of Pleurotus eryngii in vitro as well as its protective effect on alcohol-induced liver and gastric mucosa injury in vivo.Methods:The study involved determining the contents of functional components in fermented tartary buckwheat extract and observing its antioxidant capacity.Mice models of chronic alcoholic liver and gastric mucosa injury were established using Lieber-DeCarli liquid feed.The protective effects of fermented tartary buckwheat extract at low and high doses(1.5 g/kg B.W.,3.0 g/kg B.W.)were investigated for both liver and gastric mucosa injury.Results:The extract of fermented buckwheat with Pleurotus eryngii contained more antioxidant components,the contents of polyphenols,flavonoids and triterpenes were 11.40±0.32 mg GAE/g DW,17.19±0.30 mg RE/g DW and 7.59±0.24 mg/g,respectively.The contents of rutin and quercetin were as follows:13.55±0.05 and 0.665±0.01 mg/g.The iron reducing antioxidant capacity and DPPH and ABTS+free radical scavenging efficiency of Tartary buckwheat extract were 16.66±0.65,33.49±1.26 and 15.68±1.17μmol Trolox/g DW,respectively.Compared with the model group,both high-dose and low-dose groups significantly reduced malondialdehyde(P<0.05),aspartate aminotransferase(P<0.01),alanine aminotransferase(P<0.01),lactate dehydrogenase(P<0.05),and interleukin-1β(P<0.05)levels and significantly increased levels of superoxide dismutase(P<0.01)and glutathione peroxidase(P<0.01),downregulated protein expression levels of reactive oxygen species(P<0.01),rat sarcoma(P<0.01),rapidly accelerated fibrosarcoma(P<0.01),extracellular signal-regulated kinases(P<0.05),and mitogen activated protein kinase kinase(P<0.05).Conclusion:Fermented tartaty buckwheat extract of Pleurotus eryngii has good antioxidant activity,and has obvious protective effect on chronic alcoholic liver and gastric mucosa injury in mice.