摘要
[目的]鉴定分析酸奶中检出酵母,快速分析查找来源,控制产品质量,预防质量问题产生。[方法]以胀包酸奶为对象,采用孟加拉红培养基测定产品中酵母菌,分离纯化后,经菌落形态和细胞形态观察确认为酵母菌,分别采用生化鉴定及真菌种属鉴定(ITS)分离菌株。[结果]分离菌株为单一菌株,其为季也蒙假丝酵母。[结论]为生产中酸奶污染菌株的分析提供理论指导及参考方向。
[Objective]Identify and analyze the yeast detected in yogurt,quickly analyze to find the source,control product quality and prevent product quality problems.[Method]The yeast from bulged yogurt was determined by Rose Bengal Agar.After isolation and purification,the isolated strain was verified by observation of bacterial colony and cellular morphology.It was identified by physiological and biochemical identification and ITS identification respectively.[Result]The isolated strains were a single strain,which was Meyerozyma guilliermondii.[Conclusion]This study provided theoretical guidance and reference direction for the analysis of contaminated strains of yogurt in production.
作者
马静
骆敏
宋艳梅
谭莲英
范光彩
周欢
钱成林
夏忠悦
MA Jing;LUO Min;SONG Yanmei;TAN Lianying;FAN Guangcai;ZHOU Huan;QIAN Chenglin;XIA Zhongyue(New Hope Dairy Co.,Ltd.,Chengdu Sichuan 610023;Dairy Nutrition and Function,Key Laboratory of Sichuan Province,Chengdu Sichuan 610023;National Center of Technology Innovation for Dairy,Hohhot Neimenggu 010020)
出处
《中国乳业》
2024年第4期51-55,共5页
China Dairy
基金
四川省科技计划资助,优质乳制品产业化关键技术集成创新与应用(2022ZHCG0129)
四川省科技计划资助,具有减肥功效的益生菌功能特性研究及发酵乳制品产业化(2023NSFSC0018)。
关键词
酸奶
胀包
生化鉴定
ITS
季也蒙假丝酵母
yogurt
bulged
biochemical identification
ITS
Meyerozyma guilliermondii
