婴幼儿配方奶粉中1,3-二油酸-2-棕榈酸甘油三酯的测定

Determination of 1,3-dioleoyl-2-palmitoylglycerol in Infant Formula Milk Powder

[目的]建立气相色谱检测婴幼儿配方奶粉中1,3-二油酸-2-棕榈酸甘油三酯的含量。[方法]婴幼儿配方奶粉样品经温水溶解、氨水皂化、乙醚石油醚提取油脂,正己烷溶解,采用Agilent DB-1HT毛细管柱分离,通过与标准甘油三酯溶液对比确定出峰位置,采用内标法定量。[结果]方法定量限为0.008 mg/100 g,线性范围良好。低、中、高3个标准添加水平的回收率为81.1%~98.4%,相对标准偏差在2.89%~7.88%。对婴幼儿配方羊奶粉、牛奶粉基质样品中1,3-二油酸-2-棕榈酸甘油三酯的含量测定RSD≤3.03%。[结论]该方法操作过程简单、分析周期短、灵敏度高、重复性好,适用于测定婴幼儿配方牛奶粉、婴幼儿配方羊奶粉中1,3-二油酸-2-棕榈酸甘油三酯的含量,具有理想的定量效果及应用前景。

[Objective]Establish a gas chromatography method to determinate the content of 1,3-dioleoyl-2-palmitoylglycerol in infant formula.[Method] The infant formula samples were dissolved in warm water,saponified with ammonia,extracted oil with ethyl ether and petroleum ether,dissolved in n-hexane,separated by DB-1HT capillary column,determined the content of 1,3-dioleoyl-2-palmitoylglycerol by comparison with standard triglyceride solution,and quantified by internal standard method.[Result]The quantitative limit of method was 0.008mg/100 g with good linear range.The recoveries of low,medium and high standard addition levels were 81.1% ~98.4%,and the relative standard deviations were 2.89%~ 7.88%.RSD of 1,3-dioleoyl-2-palmitoylglycerol in the matrix samples of infant formula sheep milk powder and cow milk powder was determined to be less than 3.03%.[Conclusion]This method has the advantages of simple operation,short analysis period,high sensitivity and good repeatability.It is suitable for the determination of 1,3-dioleoyl-2-palmitoylglycerol content in infant formula cow milk powder and sheep milk powder,and has ideal quantitative effect and application prospect.