不同产地苦瓜干抗氧化活性成分比较分析

Comparative analysis of antioxidant active components in dried bittergourd from different habitats

测定10批苦瓜干所含的主要成分含量以及抗氧化活性,并采用傅里叶变换红外光谱法对苦瓜干进行红外光谱分析,为控制苦瓜干质量、保证其安全使用和后期开发利用提供理论支持。使用硝酸铝显色法、福林酚法和二硝基水杨酸法测定总黄酮、总酚和还原糖含量,并运用1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力法及总抗氧化能力法测定苦瓜干的抗氧化活性。10批苦瓜干的成分含量有所不同,总黄酮、总酚、还原糖含量(w)最高,分别为1.84、5.24、9.00 mg·g^(-1);苦瓜干对DPPH+自由基均有清除作用,IC_(50)值最佳为3.42 mg·m L^(-1);苦瓜干的总抗氧化能力最高,为192.02μg·g^(-1)。苦瓜干粉末的红外光谱图的主要吸收峰均在3290、2920、1740、1610、1370、1230、1020 cm^(-1)附近。苦瓜干富含黄酮、总酚、还原糖成分,一维红外光谱和二阶导数光谱的结合可为苦瓜干的识别分析提供科学数据,有助于苦瓜干的整体质量控制以及有效成分定性分析。

The main components content and antioxidant activity of 10 groups dried bitter gourd from different habitats were determined,and the dried bitter gourd was analyzed by Fourier transform infrared spectroscopy,which provided theoretical support for controlling the quality of dried bitter gourd,ensuring its safe use and later development and utilization.In this experiment,the content of total flavonoids,total phenols and reducing sugars were determined by aluminum nitrate chromogenic method,folin phenol method and dinitrosalicylic acid method,and the antioxidant activity of dried bitter gourd was determined by 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)radical scavenging ability and iron ion reducing ability(FRAP)method.The content of total flavonoids,total phenols and reducing sugar in dried bitter gourd were 1.84,5.24 and 9.00 mg·g^(-1),respectively.The dried bitter gourd can scavenge DPPH+,and the best IC_(50)value is 3.42 mg·mL^(-1).The highest total antioxidant capacity of dried bitter gourd was 192.02μg·g^(-1).The main absorption peaks of the infrared spectrum of the dry powder of bitter gourd are all around 3290,2920,1740,1610,1370,1230 and 1020 cm^(-1).The dried bitter gourd is rich in flavonoids,total phenols and reducing sugars.The combination of one-dimensional infrared spectrum and second derivative spectrum can provide scientific data for the identification and analysis of dried bitter gourd,which is helpful for the overall quality control and qualitative analysis of effective components.