摘要
以山葡萄为砧木可提高葡萄的耐寒性,但其分子调控机制还不明确。本研究以山葡萄为砧木的‘黑比诺’异嫁接苗(Vv_Va)和‘黑比诺’自嫁接苗(Vv_Vv)为材料,利用转录组数据分析两者在低温胁迫下的基因表达差异。结果表明,低温胁迫下,与自接相比,异接中显著上调的基因数330个,显著下调的基因数190个;对差异表达基因的GO富集分析表明,自接和异接的差异表达基因与非生物胁迫相关,尤其是应答温度胁迫相关基因的表达产生了显著差异;在所有差异表达的基因中,有27个热激蛋白基因、3个渗透调节物质合成关键酶基因和6个活性氧清除关键基因的表达显著上调,且有WRKY、NAC、MYB、HSF、AP2/ERF、Zinc Finger和MYC 7个家族共23个转录因子发生了差异表达,这可能是Vv_Va在遭遇低温胁迫时,抗寒砧木嫁接对接穗具有更好的保护作用。本研究通过对低温胁迫下自接苗和异接苗相关基因的转录表达差异分析,为阐释山葡萄嫁接提高葡萄耐寒性的分子机制奠定了基础。
Grafting with Vitis amurensis as rootstock can improve the cold tolerance of grapevine,but its molecular regulation mechanism is still unknown.In this study,Using heterografted seedlings with Vitis amurensis as rootstock and homografted seedlings of'Pinot Noir'as materials,transcriptome data were used to analyze the differences in gene expression between the two groups under low temperature stress.The results showed that 330 genes were significantly up-regulated and 190 genes were significantly down-regulated in heterografted seedlings.GO enrichment analysis showed that the DEGs between heterografted and homografted seedlings were related to abiotic stress,especially to temperature stress.Among all DEGs,27 heat shock protein genes,three key enzyme genes for the synthesis of osmoregulatiory substances and six key genes of ROS scavenging were significantly upregulated.And seven gene families including 23 transcription factors,such as WRKY,NAC,MYB,HSF,AP2/ERF,Zinc Finger and MYC were differential expressed,which may play an important role in protecting and regulating the scions under chilling stress.Through the analysis on DEGs between heterografted and homografted seedlings under chilling stress,it will provide a basis for explaining the molecular mechanism of improving the cold tolerance of grapevine heterografted seedlings using vitis amurensis as rootstock.
作者
徐美隆
王毅
牛锐敏
许泽华
黄小晶
陈卫平
XU Meilong;WANG Yi;NIU Ruimin;XU Zehua;HUANG Xiaojing;CHEN Weiping(Horticulture Institute,Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan 750002,China;Institute of Botany,the Chinese Academy of Sciences,Beijing 100093,China)
出处
《中外葡萄与葡萄酒》
北大核心
2024年第3期22-29,共8页
Sino-Overseas Grapevine & Wine
基金
宁夏回族自治区自然科学基金(2022AAC03440)
宁夏回族自治区农业育种专项(NXNYYZ202101-03)财政部和农业农村部:国家现代农业产业技术体系(CARS-29-28)。
关键词
山葡萄
嫁接
低温胁迫
转录调控
Vitis amurensis
grafting
chilling stress
transcriptional regulationn