底质对硬壳蛤(Mercenaria mercenaria)幼贝潜砂及相关酶活性的影响

The Effect of Substrate on the Burrowing and Related Enzyme Activities of Juvenile Hard Clams Mercenaria mercenaria

为研究底质对硬壳蛤幼贝潜砂行为及相关酶活性的影响,设置5种底质,观察幼贝的潜砂过程,统计潜砂时间及潜砂率,测定呼吸代谢酶活性。结果表明:硬壳蛤幼贝潜砂率排序为泥组<粗砂泥组<细砂泥组<粗砂组<细砂组;10%硬壳蛤幼贝竖壳时间排序为粗砂组<细砂组<细砂泥组<泥组<粗砂泥组;10%硬壳蛤幼贝潜砂时间排序为粗砂组<细砂组<粗砂泥组<细砂泥组<泥组;Na^(+)-K^(+)-ATP酶活性排序为粗砂泥组<细砂组<粗砂组<泥组<细砂泥组;Ca^(2+)-Mg^(2+)-ATP酶活性排序为粗砂泥组<粗砂组<细砂组<细砂泥组<泥组;乳酸脱氢酶活性排序为细砂组<粗砂组<粗砂泥组<泥组<细砂泥组;琥珀酸脱氢酶活性排序为粗砂泥组<细砂泥组<泥组<细砂组<粗砂组。综合分析,细砂底质更适合于作为硬壳蛤幼贝养殖的底质。

In order to study the effect of substrate on the burrowing behavior and related enzyme activities of juvenile hard clam,five kinds of substrates were set up to observe the burrowing process of juvenile hard clam,count the burrowing time and burrowing rate,and determine the activity of respiratory metabolic enzymes.The results showed that the burrowing rate of juvenile hard clam was mud group<coarse sand mud group<fine sand mud group<coarse sand group<fine sand group.The erect shell ET10 was coarse sand group<fine sand group<fine sand mud group<mud group<coarse sand mud group;The burrowing ET10 was coarse sand group<fine sand group<coarse sand mud group<fine sand mud group<mud group.Na^(+)-K^(+)-ATPase activity was coarse sand mud group<fine sand group<coarse sand group<mud group<fine sand mud group;Ca^(2+)-Mg^(2+)-ATPase activity was coarse sand mud group<coarse sand group<fine sand group<fine sand mud group<mud group.Lactate dehydrogenase activity was fine sand group<coarse sand group<coarse sand mud group<mud group<fine sand mud group.Succinate dehydrogenase activity was coarse sand mud group<fine sand mud group<mud group<fine sand group<coarse sand group.Fine sand substrate was the most suitable substrate for the culture of juvenile hard clams according to a comprehensive analysis.