复杂染料废水降解过程中生物炭对Aeromonas hydrophila菌的影响

Effect of Biochar on the Degradation of Complex Dye Wastewater by Aeromonas hydrophila

生物降解复杂染料废水过程中细菌活性是有效降解的关键。本研究分离驯化出染料废水降解菌,并对其进行形态学特征和系统发育的分析鉴定,将生物炭及Fe_(2)O_(3)/生物炭(Fe-BC)分别与菌构建复合体系,研究降解过程中生物炭和Fe-BC对菌的影响。结果表明,该降解菌为Aeromonashydrophila(嗜水气单胞菌菌属),生物炭-菌和Fe-BC-菌体系中降解脱色率分别提高18%和75%,降解脱色过程符合准一级动力学模型。在Fe-BC-菌体系中,Fe-BC可以促进菌细胞的代谢和细胞间的物质传递,使得参与代谢的N、P、S、Ca、Na及Fe等元素含量增加,并促使细胞产生胞外分泌物。Fe^(2+)参与菌降解复杂有机物的过程,降解产物主要有N-2-(3,4-二羟苯基)-乙基甲胺、2,3-二氢-1,3-二氧-1H-异吲哚-5-羧酸(含C=O)及甲氧基乙酸甲酯(含C—O)。生物炭与Fe-BC可以提高嗜水气单胞菌的生物活性,完成对复杂染料废水的降解脱色.

The activity of bacteria is the key to effective degradation for complex dye wastewater.In this study,dye wastewater degrading bacteria were isolated and domesticated,Their morphological characteristics and phylogenetic evolution were analysed and identified.A composite system was constructed by biochar and Fe_(2)0_(3)/biochar(Fe-BC)with the bacteria to study the effects of biochar and Fe-BC on the bacteria during the degradation process.The results showed that the degrading bacteria was Aeromonas hydrophila,and the degradation and decolourisation rates increased by 18%and 75%in the biochar-bacteria and Fe-BC-bacteria systems,respectively.The degradation and decolourisation process followed the first-order degradation kinetic model.In the Fe-BC-bacteria system,the metabolism of bacterial cells and material transfer between cells were promoted,the content of elements involved in metabolism such as N,P,S,Ca,Na and Fe were increased.Fe^(2+) was involved in the process of complex organic matter and the degradation products mainly included N-2-(3,4-dihydroxyphenyl)-ethylmethylamine,2,3-dihydro-1,3-diox-1H-isoindole-5-carboxylic acid(C=0)and methyl methoxyacetate(C-O).Biochar and Fe-BC could enhance the biological activity of Aeromonas hydrophila and completed the degradation and decolourisation of complex dye wastewater.