陕北白绒山羊隐性乳房炎致病菌分离鉴定与药敏试验分析

Isolation,Identification and Drug Sensitivity Test of Recessive Mastitis Pathogenic Bacteria in Northern Shaanxi White Cashmere Goat

为调查陕北白绒山羊隐性乳房炎流行情况,制定科学的防控策略。试验于2023年从30个陕北白绒山羊养殖场随机采集378份乳样,采用加州乳房炎检测法(CMT)和体细胞计数法对乳样进行隐性乳房炎检测,对阳性样品中细菌进行分离鉴定与药敏试验。结果显示,共54份乳样隐性乳房炎阳性,阳性率为14.29%(54/378);从54份阳性乳样中共分离获得31株细菌,分别为葡萄球菌、大肠杆菌和链球菌,所占比例分别为64.52%(20/31)、22.58%(7/31)和12.90%(4/31);3种分离的菌株对新霉素、氟苯尼考、诺氟沙星、头孢噻呋和头孢西丁均敏感。该调查结果说明,陕北白绒山羊隐性乳房炎发病率较高,养殖场(户)需采取合理用药妥善做好该病的防治。

In order to investigate the prevalence of recessive mastitis of northern Shaanxi white cashmere goat,and formulate scientific prevention and control strategies.378 milk samples were randomly collected from 30 northern Shaanxi white cashmere goat farms in 2023,and recessive mastitis was detected by California mastitis test(CMT)and somatic cell counting.The bacteria in the positive samples were isolated,identified and tested for drug sensitivity.The results showed that 54 samples were positive for recessive mastitis,and the positive rate was 14.29%(54/378).A total of 31 strains of bacteria were isolated from the 54 positive milk samples,includingStaphylococcus,EscherichiacoliandStreptococcus,accounting for 64.52%(20/31),22.58%(7/31)and 12.90%(4/31)respectively.The three kinds of isolated strains of bacteria were sensitive to neomycin,florfenicol,norfloxacin,ceftiofur and cefoxitin.It indicates that the incidence of recessive mastitis of northern Shaanxi white cashmere goat is high,and breeding farms(households)should prevent and control this disease by using rational drug properly.