UHPLC-Q-TOF-MS检测医院制剂胃特安片主要化学成分及入血成分

Determination of Chemical Components and Blood Entering Components of Weite’an Tablets Hospital Preparations Based on UHPLC-Q-TOF-MS

目的采用UHPLC-Q-TOF-MS对医院制剂胃特安片的化学成分进行分析和鉴定,并寻找大鼠血浆中的药物移行成分。方法0.05%甲酸水-乙腈流动相体系下,Acquity UPLC BEH C_(18)色谱柱色谱分离后进行Q-TOF正负离子扫描,SWATH采集模式下进行数据处理,定性鉴定的同时通过MRMHR数据处理功能进行血浆样品中各成分的定量分析研究。结果实验中共分离鉴定出52种化学成分,黄酮类成分22种,酚酸类6种,萜类化合物5种,香豆素类3种,生物碱类2种以及氨基酸等其他类化合物。检出8种入血成分:6-羟基-7-甲氧基香豆素、橙皮素、川陈皮素、橘皮素、水苏碱、小檗碱、汉黄芩苷及槲皮苷。结论该方法准确稳定,适用于胃特安片剂中化学成分分析与鉴定研究,实验结果为临床合理用药、后续药物组织分布及效应物质基础研究提供基础。

OBJECTIVE To analyze and identify the chemical components of Weite’an tablet which is a hospital preparation by UHPLC-Q-TOF-MS,and to find the drug migration components in rat plasma.METHODS In 0.05%formic acid water acetonitrile mobile phase system,after chromatographic separation of the Acquire UPLC BEH C_(18)column,Q-TOF positive and negative ion scanning was performed,and data processing was performed in SWATH collection mode.At the same time,quantitative analysis of various components in plasma samples was conducted through MRMHR data processing function during qualitative identification.RESULTS A total of 52 chemical components were isolated and identified in the experiment,including 22 flavonoids,6 phenolic acids,5 terpenoids,3 coumarins,2 alkaloids,amino acids and other compounds.Eight components were introduced into the blood:6-hydroxy-7-methoxycoumarin,hesperidin,tangerine,stachydrine,berberine,scutellarin and quercetin.CONCLUSION This method is accurate and stable,and is suitable for the analysis and identification of chemical components in Weite’an tablets.The experimental results lay a solid foundation for clinical rational drug use,follow-up drug tissue distribution and basic research of effectors.