一步电沉积法制备导电Cu-MOF检测左氧氟沙星

Preparation of conductive Cu-MOF for levofloxacin determination by one-step electrodeposition

采用一步电化学沉积法在玻碳电极(Glassy Carbon Electrode,GCE)表面沉积了铜基导电金属有机框架(Metal-organic Frameworks,MOF)材料,制备了Cu-MOF修饰的电极(Cu-MOF/GCE),考察了Cu-MOF/GCE的电化学性能和它对左氧氟沙星的检测效果.结果表明,该修饰电极对左氧氟沙星的氧化过程具有明显的电催化作用.利用循环伏安法(Cyclic Voltammetry,CV)考察了沉积电压、沉积时间、pH值和扫速对左氧氟沙星电化学行为的影响,结果表明最佳的沉积电压为-1.3 V,沉积时间为300 s,缓冲溶液pH为7.5.在优化后的条件下用差分脉冲伏安法(Differential Pulse Voltammetry,DPV)研究了左氧氟沙星的氧化峰电流值与浓度之间的关系,得出其线性响应范围为25~300μmol/L,检出限达到7.25μmol/L(3 S/N),对左氧氟沙星滴眼液和加标湖水中的回收率为96.1%~112.5%.该电极制备方法简单,灵敏度高,抗干扰性强,为实际样品中左氧氟沙星的测定提供了一种新方法.

In this paper,a Cu-based conductive metal organic frame(Metal-organic framework,MOF)was deposited on the surface of a glass carbon electrode(Glassy carbon electrode,GCE)resulting in a Cu-MOF modified electrode(Cu-MOF/GCE).We investigated the electrochemical properties of Cu-MOF/GCE and its detection of levofloxacin.The results demonstrated that the modified electrode exhibits significant electrocatalysis for the oxidation of levofloxacin.The influence of deposition voltage,deposition time,pH and scan rates on the electrochemical behavior of levofloxacin was also studied by cyclic voltammetry(Cyclic voltammetry,CV),the results displayed that the optimal deposition voltage is-1.3V,the deposition time is 300 s,and the buffer solution pH is 7.5.The relationship between the oxidation peak current value and concentration of levofloxacin was investigated by using the differential pulse voltammetry(Differential pulse voltammetry,DPV),the result showed that the linear response range was 25 to 300μmol/L,and the detection limit reached 7.25μmol/L(3S/N).The recovery rate of levofloxacin eye drops and spiked lake water was 96.1%~112.5%.The Cu-MOF/GCE preparation method is simple,sensitive,and interference-resistant,which provides a new method for the determination of levofloxacin in real samples.