斑马鱼幼鱼在中药单体毒性检测的研究进展

Research Progress on Toxicity Detection of Chinese Medicine Monomer in Zebrafish Larvae

中药是多成分、多靶点的复杂体系,是我国几千年文明积累下来的宝贵资源,但由于中药的毒性研究缺乏,导致其使用安全范围不明确,限制了其广泛使用和推广,故对每种中药单体的毒性评价尤为重要。斑马鱼是一种重要的脊椎动物模型,用于低成本的体内全生物中通量药物筛选和毒理研究。综述了利用斑马鱼检测多种中药单体的心脏毒性、肝毒性、神经毒性和发育毒性的研究进展,指出了其在中药单体毒性研究中存在的问题。斑马鱼是中药单体毒性及安全性评价的理想模式生物,未来,需建立标准规范和高通量测定方法对上千种中药单体进行安全性评价。

Traditional Chinese medicine(TCM)is a complex system of multi-components and multi-targets,which is a precious resource accumulated by Chinese civilization for thousands of years.However,due to the lack of toxicity studies on TCM,the safe range of its use is unclear,which limits its wide use and promotion.Therefore,the toxicity evaluation of each TCM monomer is particularly important.Zebrafish is an important vertebrate model for low-cost in vivo whole-organism midthroughput drug screening and toxicology studies.The study reviews the research progress of the detection of cardiotoxicity,hepatotoxicity,neurotoxicity and developmental toxicity of various TCM monomers by zebrafish;and points out the existing problems in the study of toxicity of TCM monomers.Zebrafish is an ideal model organism for toxicity and safety evaluation of traditional Chinese medicine monomers.In the future,standards and high-throughput determination methods should be established to evaluate the safety of thousands of traditional Chinese medicine monomers.