9个燕麦品种的种带细菌多样性及其促生功能定性分析

Qualitative analysis of seed-borne bacterial diversity and growth-promoting function of nine oat(Avena sativa)varieties

【目的】探究燕麦(Avena sativa)种带细菌及其促生功能多样性。【方法】采用传统培养方法分离纯化9个燕麦品种种带细菌,并定性分析其固氮、溶磷、产IAA能力和分解纤维素功能。【结果】共分离到34株细菌分离物,结合其形态特征及16S rDNA分析,分离出的细菌隶属于原核生物界的4门19属,其中优势门为厚壁菌门(Firmicutes),占比56.00%;优势属为芽孢杆菌属(Bacillus),占比41.18%;34株燕麦种带细菌均具不同的促生功能,其中分属于芽孢杆菌属、埃希氏菌属(Escherichia)、假单胞菌属(Pseudomonas)、志贺氏菌属(Shigella)和大西洋杆菌属(Atlantibacter)的9株细菌同时具备固氮、溶磷、产吲哚乙酸和分解纤维素功能。【结论】本试验共分离到34株细菌分离物,均具备不同的促生功能,其中9株细菌兼具固氮、溶磷、产吲哚乙酸和分解纤维素功能,志贺氏菌属LFt4综合能力最强。

【Objective】In order to explore the diversity of seed-borne bacteria of oat and their growth-promoting functions.【Method】9 seed-borne bacteria of oat cultivars were isolated and purified by traditional cul⁃ture methods,and their functions of nitrogen fixation,phosphorus solubilization,IAA production and cellulose decom⁃position were qualitatively analyzed.【Result】A total of 34 bacterial isolates were isolated.Combined with their mor⁃phological characteristics and 16S rDNA analysis,the isolated bacteria belonged to 4 phyla and 19 genera of prokary⁃otes,in which Firmicutes was the dominant phyla for 56.00%.The dominant genus was Bacillus sp.,accounting for 41.18%.34 strains of oat seed bacteria all had different growth⁃promoting functions.It belonged to the Bacillus sp.,Escherichia sp.,Pseudomonas sp.,Shigella sp.,and Atlantibacter sp.9 strains had the functions of nitrogen fixation,phosphorus solubilization,indole-acetic acid production and cellulose decomposition.【Conclusion】A total of bacte⁃rial 34 isolates were isolated in this experiment,all of which had different growth-promoting functions.Among them,9 strains had the functions of nitrogen fixation,phosphorus solubilization,indoleacetic acid production and cellu⁃lose decomposition.Shigella sp.LFt4 had the strongest comprehensive ability.