基于PI3K/Akt/mTOR信号通路探讨乌苏酸对结肠癌细胞的影响

Exploring the mechanism of ursolic acid in the treatment of colon cancer based on the PI3K/Akt/mTOR signaling pathway

目的:探讨乌苏酸(UA)对结肠癌细胞的影响及其作用机制。方法:设立空白对照组及不同浓度UA给药组,采用CCK-8法测定各组细胞的增殖活力,通过细胞划痕实验测定各组细胞的迁移能力及通过Western blot法测定UA对PI3K/Akt/mTOR信号通路的影响。结果:CCK-8实验表明UA能明显降低SW480及LOVO结肠癌细胞生存率,且随着药物浓度的升高,细胞的生存率显著降低(P<0.05)。细胞划痕实验表明,与对照组相比,低浓度的UA处理的细胞愈合率降低不明显(P>0.05),而高浓度的UA处理的细胞的愈合率明显降低(P<0.05)。Western blot实验结果显示,UA处理过的细胞p-PI3K、p-Akt、p-mTOR蛋白表达量明显下降(P<0.05)。结论:UA可通过抑制PI3K/Akt/mTOR信号通路的表达,从而抑制结肠癌细胞增殖、迁移,具有明确的抗肿瘤性。

Objective:To investigate the effect of ursolic acid on the colon cancer cells and its mechanism.Methods:A blank control group and different concentrations of ursolic acid treatment groups were established.The proliferation activity of each group of cells was measured using the CCK-8 method.The migration ability of each group of cells was measured through cell scratch assay,and the effect of ursolic acid on the PI3K/Akt/mTOR signaling pathway was measured through WB assay.Results:Through CCK-8 experiments,it was found that ursolic acid could significantly reduce the survival rate of SW480 and LOVO colon cancer cells,and with the increase of drug concentration,the survival rate of cells significantly decrease(P<0.05).The cell scratch experiment showed that compared with the control group,the low concentration of ursolic acid treatment did not significantly reduce the cell healing rate(P>0.05),while the high concentration of ursolic acid treatment significantly reduced the cell healing rate(P<0.05).The WB experiment results showed that the expression levels of p-PI3K,p-Akt,and p-mTOR proteins in cells treated with ursolic acid were significantly reduced(P<0.05).Conclusion:Ursolic acid has clear anti-tumor properties by inhibiting the expression of the PI3K/Akt/mTOR signaling pathway,thereby inhibiting the proliferation and migration of colon cancer cells.