印度梨形孢-花生共生体系建立及对花生的促生作用

Establishment of Piriformospora indica-peanut symbiosis system and its promoting effect on peanut growth

印度梨形孢(Piriformospora indica)是一种内生真菌。为提高花生产量,探究印度梨形孢对花生的促生作用,以花生(粤油7号)为材料,采用室外盆栽实验,建立印度梨形孢与花生共生体系,调查对花生植株的农艺性状、产量、光合特性、丙二醛含量、根系活力和抗氧化酶活性等生理指标的影响。结果发现:采用菌丝浇灌的方式接种印度梨形孢,成功建立了与花生的共生体系,侵染率为63%。苗期时,叶片叶绿素SPAD值和净光合速率显著增加24.17%和19.66%,蒸腾速率比未接种印度梨形孢的花生植株22%;在苗期与成熟期,接种印度梨形孢的花生主根长、地下部鲜重、地下部干重、根系活力和叶片的过氧化氢酶(CAT)、过氧化物酶(POD)活性显著提高,丙二醛含量分别显著降低24.27%和22.12%;接种印度梨形孢提高了花生产量。

To improve peanut yield,growth-promoting effect of endophytic fungus Piriformospora indica on peanut plants was investigated in pots.Peanut cv.Yueyou 7 was used as experimental material.Symbiosis system between P.indica and peanut was established.Agronomic traits,yield,photosynthetic characteristics,malondialdehyde content,root activity and antioxidant enzyme activity of peanut were studied.Results showed that the symbiosis system of P.indica and peanut was established successfully by inoculating P.indica with hyphae.The infection rate was 63%.At seedling stage,net photosynthetic rate and chlorophyll SPAD value of peanut leaves were significantly increased by 24.17%and 19.66%,and transpiration rate was 22%lower than that of uninoculated peanut plants.At seedling and mature stages,compared with uninoculated P.indica,root length,underground fresh weight,underground dry weight,root activity and leaf catalase peroxidase activity were significantly increased after inoculation.Content of malondialdehyde was significantly reduced by 24.27%and 22.12%at seedling and mature stages.Peanut yield was increased by inoculation with P.indica.