油菜薹中啶虫脒残留质控样品的制备与评价

Preparation and evaluation of acetamiprid quality control samples in rapeseed stalk

为保障油菜薹中啶虫脒农药残留快速检测结果的准确性和可靠性,建立油菜薹中啶虫脒质控样品的制备方法并进行评价。采用基质加标、真空冷冻干燥的方法制备冻干质控样品。采用液相色谱质谱串联的检测方法对质控样品中的啶虫脒含量进行测定,通过随机抽样检验以评估样品的均匀性,通过观察质控样品在一个月内啶虫脒含量变化以评估样品的稳定性。该方法制备的油菜薹中啶虫脒农药残留质控样品均匀性和稳定性良好,便于储存和运输,适用于快检产品验证、盲样考核等快速检测领域。本研究建立了一种流程简单的油菜薹冻干粉质控样品制备方法,为蔬菜中农药残留质控样品制备共性关键技术研究提供了数据参考。

In order to ensure the accuracy and reliability of the rapid determination of acetamiprid pesticide residues in rapeseed stalk,a method for the preparation of quality control samples was established and evaluated.The lyophilized quality control samples were prepared by matrix spiking and vacuum freeze-drying.And the determination of acetamiprid in the quality control samples was carried out by liquid chromatography-tandem mass spectrometry.The uniformity of the samples was assessed by random sampling and the stability of the samples was evaluated by observing the changes of acetamiprid over a period of one month.The quality control samples prepared by this method are homogeneous and stable,easy to store and transport,and suitable for rapid testing in the field of rapid test kit validation and blind sample assessment.This study established a simple process for the preparation of lyophilised powder quality control samples of rapeseed stalk,which provides a data reference for the study of common key technologies for the preparation of quality control samples of pesticide residues in vegetables.