UPLC法同时测定外感风痧颗粒中14种成分的含量

Simultaneous content determination of fourteen constituents in Waigan Fengsha Granules by UPLC

目的建立UPLC法同时测定外感风痧颗粒中绿原酸、原儿茶酸、原儿茶醛、隐绿原酸、丁香酸、1,3-二咖啡酰奎宁酸、新绿原酸、木犀草苷、异绿原酸B、异绿原酸A、橙皮苷、咖啡酸、异绿原酸C、迷迭香酸的含量。方法分析采用Waters ACQUITY UPLC®HSS C_(18)色谱柱(2.1 mm×100 mm,1.8μm);流动相乙腈-0.2%磷酸,梯度洗脱;体积流量0.2 mL/min;柱温30℃;检测波长275、327 nm。再进行化学计量学分析。结果14种成分在各自范围内线性关系良好(r≥0.9996),平均加样回收率95.68%~104.8%,RSD 0.7%~3.0%。7批样品聚为2类,3种主成分累积贡献率达93.031%。结论该方法准确可靠,可用于外感风痧颗粒的质量控制。

AIM To establish a UPLC method for the simultaneous content determination of chlorogenic acid,protocatechuic acid,protocatechualdehyde,cryptochlorogenic acid,syringic acid,1,3-dicaffeoylquinic acid,cynaroside,isochlorogenic acid B,neochlorogenic acid,isochlorogenic acid A,hesperidin,caffeic acid,isochlorogenic acid C and rosmarinic acid in Waigan Fengsha Granules.METHODS The analysis was performed on a 30℃thermostatic Waters ACQUITY UPLC®HSS C_(18)column(2.1 mm×100 mm,1.8μm),with the mobile phase comprising of acetonitrile-0.2%phosphoric acid flowing at 0.2 mL/min in a gradient elution manner,and the detection wavelengths were set at 275,327 nm.Subsequently,chemometric analysis was performed.RESULTS Fourteen constituents showed good linear relationships within their own ranges(r≥0.9996),whose average recoveries were 95.68%-104.8%with the RSDs of 0.7%-3.0%.Seven batches of samples were clustered into two types,three principal components demonstrated the acumulative contribution rate of 93.031%.CONCLUSION This accurate and reliable method can be used for the quality control of Waigan Fengsha Granules.