高内涵无荧光标记活细胞的运动追踪分析

Motion Tracking Analysis of High-content Non-fluorescent Labelling in Living Cells

细胞运动与多种细胞行为及疾病发生治疗等息息相关,通过实时细胞追踪和分析可为揭示细胞行为以及细胞运动的规律、以及疾病发生机制等提供实验依据。当前细胞追踪的常规实验方法多是从群体层面上对细胞迁移进行间接分析,无法追踪迁移过程中单个细胞的形态学以及运动动力学等参数的变化,尤其对于无荧光标记活细胞,常规明场或相差图像的追踪分析,存在图像中背景与细胞间的对比度低、细胞边界不连续等问题,难以实现细胞形态的准确识别和分辨以及自动追踪分析。高内涵细胞成像系统设备结合了活细胞成像模块和多参数的高内涵分析软件,可实现无标记活细胞快速成像以及自动分割识别和追踪分析。本文总结了高内涵成像系统的无标记细胞分析模块在体外细胞运动追踪实验中的应用。

Cell movement is closely related to a variety of cell behaviors and disease treatment.Real-time cell tracking and analysis can provide experimental basis for revealing cell behavior,cell movement rules,and disease mechanism.At present,the conventional experimental methods of cell tracking are mostly indirect analysis of cell migration from the group level,which cannot track the changes of parameters such as morphology and motion dynamics of single cells during migration.Especially for non-fluorescent labelling in living cells,the tracking analysis of conventional bright field or phase difference images has problems such as low contrast between the background and cells in the image and discontinuous cell boundaries.It is difficult to achieve accurate identification and resolution of cell morphology and automatic tracking analysis.The high-content imaging system equipment combines the live cell imaging module and multi-parameter high-content analysis software,which can realize fast imaging of unlabeled live cells and automatic segmentation,recognition and tracking analysis.This paper summarizes the application of the label-free cell analysis module of the high-content imaging system in in vitro cell movement tracking experiments.