水蛭对局灶节段性肾小球硬化大鼠足细胞损伤的影响

Effects of Shuizhi(Leeches)on Podocyte Injury in Focal Segmental Glomerulosclerosis Rats

目的:观察水蛭对局灶节段性肾小球硬化(focal segmental glomerulosclerosis,FSGS)模型大鼠足细胞损伤的影响。方法:44只大鼠随机分为正常组、模型组、水蛭组3组,采用单侧肾切除术后尾静脉注射阿霉素法建立大鼠FSGS模型,水蛭组给予水蛭悬浊液0.9 g/(kg·d)灌胃,正常组、模型组予等量生理盐水灌胃,共8周。分别于给药后4周、8周末测定大鼠血清三酰甘油(TG),免疫荧光法、Western Blot法检测肾组织线粒体融合蛋白2(Mfn2)蛋白表达,PCR法检测Mfn2 mRNA表达。结果:水蛭组大鼠血TG水平较模型组降低(P<0.05);肾组织Mfn2蛋白和Mfn2 mRNA表达较模型组大鼠增多(P<0.05)。结论:水蛭可以降低FSGS大鼠TG水平,提高Mfn2蛋白及mRNA表达水平,减少足细胞损伤。

Objective:To observe the effects of Leeches on podocyte injury in rats with focal segmental glomerulosclerosis(FSGS).Methods:44 rats were randomly divided into three groups:normal group,model group,and Leech group.FSGS model was established by Doxorubicin injection in the tail vein after unilateral nephrectomy.Leeches group was given Leeches suspension 0.9 g/(kg·d),normal control and model groups were given equal amount of saline,they were medicated for 8 weeks.Blood triglyceride(TG)was determined,immunofluorescence,Western blot were used to determine Mfn2 protein expression and Mfn2 mRNA was measured by PCR after administration for 4 weeks and 8 weeks,respectively.Results:The TG of the rats in the Leeches group was lower than that in the model group(P<0.05).The expressions of Mfn2 protein and Mfn2 mRNA in the Leeches group were higher than those in the model group(P<0.05).Conclusion:Leeches can reduce the level of TG,increase the expression levels of Mfn2 protein and Mfn2 mRNA,reduce podocyte damage.