玄参“发汗”前后对小鼠耳肿胀及腹腔通透性影响

Effect of ear swelling and abdominal permeability before and after"sweating"in mice

目的:对比分析玄参“发汗”前后对小鼠耳肿胀及腹腔通透性影响,为玄参的加工、临床应用和进一步研究提供参考。方法:采用二甲苯致小鼠耳廓肿胀分析玄参“发汗”前后对耳廓肿胀抑制率,小鼠尾静脉注射伊文思蓝,腹腔注射醋酸造模,测定吸光度。建立抗炎模型,以玄参“发汗”前后水提取物进行药物干预。采用SPSS 26.0统计分析软件分析处理,数据用x±s表示,两组之间的比较用Dunnett t检验,P<0.05为差别具有统计学意义。评价玄参“发汗”前后抗炎作用的药效学差异。结果:玄参“发汗”前高剂量组与生理盐水组作比较,P<0.05差异具有显著性,低、中、高剂量组抑制率分别为8.65%、2.61%、17.08%,玄参“发汗”后低剂量组与生理盐水组作比较P<0.01有统计学意义,高、中剂量组与生理盐水组作比较,P<0.05差异具有显著性,低、中、高剂量组抑制率分别为27.03%、23.99%、39.84%;玄参“发汗”前后高中低剂量组与生理盐水组比较P<0.01,差异具有统计学意义;均能够抑制小鼠腹腔毛细血管通透性。结论:“发汗”引起玄参中化学成分含量变化、抗炎效果增强。

Objective:To analyze the effects of ear swelling and peritoneal cavity permeability in mice,and to provide reference for its processing,clinical application and further research.Methods:The injection of the ear cage swelling,and the absorbance.An anti-inflammatory model was established,and the water extract before and after“sweating”was used for drug intervention.Analyzed and processed using SPSS 26.0 statistical analysis software,data were expressed as x±s and comparison between two groups were tested by Dunnett t test,P<0.05 considered differential statistically significant.To evaluate the pharmacodynamic differences in the anti-inflammatory effects of the shen before and after“sweating”.Results:Compared with the high dose group and the normal saline group,The P<0.05 difference was significant,The inhibition rates in the low-,medium-,and high-dose groups were 8.65%,2.61%,and 17.08%,respectively,P<0.01 between the low dose group and the saline group after“sweating”,The high and medium dose groups were compared to the saline group,The P<0.05 difference was significant,The inhibition rates in the low,medium and high dose groups were 27.03%,23.99% and 39.84%,respectively;P<0.01 between high and low dose and saline groups before and after sweating,The difference was statistically significant;Are able to inhibit the peritoneal capillary permeability in mice.Conclusion:“sweating”causes the change of chemical content in xuanginseng and the anti-inflammatory effect.