男性不育症患者精子DNA碎片指数检测意义

Study on the significance of sperm DNA fragmentation index in male infertility patients

目的分析男性不育症患者精子DNA碎片指数(DFI)检测意义。方法选定本院2021年5月至2023年5月就诊的150例男性不育症患者设为观察组,另选取同期本院150例孕前体检并于随访1年内女方受孕者设为对照组,采集精液标本,检测、比较2组DFI、精液参数,根据DFI将男性不育症患者分为3组,DFI≤15%的36例患者设为A组,DFI在>15%~30%的64例患者设为B组,DFI>30%的50例患者设为C组,比较3组精液参数,Pearson分析DFI与精液参数的相关性。结果观察组DFI高于对照组(P<0.05)。观察组前向运动精子百分率(PR)、精子浓度、精子正常形态率、精子存活率均低于对照组(P<0.05)。C组PR、精子浓度、精子正常形态率、精子存活率均低于B组、A组(P<0.05),B组PR、精子浓度、精子正常形态率、精子存活率均低于A组(P<0.05)。DFI与PR、精子浓度、精子正常形态率、精子存活率均呈负相关性(r=-0.403、-0.381、-0.376、-0.396,P均<0.001)。结论男性不育症患者DFI较高,DFI与精液参数存在一定的相关性,可作为评估患者病情的重要指标。

Objective To analyze the significance of sperm DNA fragmentation index(DFI)detection in male infertility patients.Methods A total of 150 male infertility patients treated in our hospital from May 2021 to May 2023 were selected as the observation group,and 150 female pregnant patients who underwent pre-pregnancy physical examination in our hospital during the same period and were followed up for one year were selected as the control group.Semen samples were collected,DFI and semen parameters of the two groups were detected and compared,and male infertility patients were divided into 3 groups according to DFI.Thirty-six patients with DFI≤15%were assigned as group A,64 patients with DFI>15%~30%were assigned as group B,and 50 patients with DFI>30%were assigned as group C.Semen parameters of the three groups were compared,and the correlation between DFI and semen parameters was analyzed by Pearson.Results DFI in observation group was higher than that in control group(P<0.05).The percentage of forward motile sperm(PR),sperm concentration,sperm normal form rate and sperm survival rate in observation group were lower than those in control group(P<0.05).The PR,sperm concentration,sperm normal form rate and sperm survival rate of group C were lower than those of groups B and A(P<0.05),respectively and the PR,sperm concentration,sperm normal form rate and sperm survival rate of group B were lower than those of group A(P<0.05).DFI was negatively correlated with PR,sperm concentration,sperm normal morphology rate and sperm survival rate respectively(r=-0.403,-0.381,-0.376,-0.396,P<0.001).Conclusion DFI is higher in male infertility patients,and there is a certain correlation between DFI and semen parameters,which can be used as an important indicator to evaluate the patient′s condition.