摘要
目的分析在梅毒检测中化学发光法与甲苯胺红不加热血清试验2种方式的实际应用价值。方法采用回顾性研究方式,对逆向梅毒筛查流程实验室数据进行分析,对2022年1月至2023年6月入院的867例患者进行化学发光法进行抗体筛查试验,检测显示阳性样本实施非梅毒螺旋体抗原血清甲苯胺红不加热试验检测进行复测,分析2种检测方法对梅毒检出结果。结果在867例样本中,采用化学发光法检测显阳性样本6例,对6例阳性样本采用甲苯胺红不加热试验进行复测,结果呈阳性4例,阴性2例。化学发光法标本的吸光度与临界值的比值(S/CO)>10时,采用甲苯胺红不加热血清试验具有较高的复查阳性率。结论梅毒检测中化学发光法的检出率较高,已经确诊的梅毒患者需要甲苯胺红不加热血清试验进行滴度测定,来观察疗效、随访及监测有无复发等。综合使用多种检测方法,可以提高梅毒的检测的准确性,更好地指导患者的治疗和管理。
Objective To analyze the practical application value of chemiluminescence method and tolui-dine red unheated serum test in syphilis detection.Methods A retrospective study was conducted to analyze the laboratory data of the reverse syphilis screening process.867 patients admitted from January 2022 to June 2023 were tested for antibody screening by chemiluminescence method.The positive samples were retested by non-Tre-ponema pallidum antigen serum Toluidine Red unheated test.The detection results of syphilis by the two methods were analyzed.Results Among the 867 samples,6 cases were positive by chemiluminescence detection,and 6 cases were retested by toluidine red unheated test.The results were positive in 4 cases and negative in 2 cases.When the S/CO value of the chemiluminescence method was>10,the toluidine red unheated serum test had a high positive rate of re-examination.Conclusion The patients underwent antibody screening test by chemilumines-cence method,and the positive samples were detected by non-Treponema pallidum antigen serologic test.The de-tection rate of chemiluminescence method is high.The confirmed syphilis patients still need toluidine red unheat-ed serum test for titer determination to observe the curative effect,follow-up and monitor whether there is recur-rence.A variety of detection methods are used comprehensively,it can improve the accuracy of syphilis detection and better guide the treatment and management of patients.
作者
谢乔芬
赖谋忠
杨未琴
Xie Qiaofen;Lai Mouzhong;Yang Weiqin(Department ofLaboratory Medicine,the Second People′s Hospital,Jinggangshan 343609,China)
出处
《实用医技杂志》
2024年第4期278-280,共3页
Journal of Practical Medical Techniques
关键词
梅毒
发光测定法
甲苯胺红不加热血清试验
阳性率
Syphilis
Luminescent measurements
Toluidine red unheated serum test
Positive rate